Technical datasheet
Description
The monoclonal antibody ED1 recognises a single chain heavily glycosylated protein of 90-110 kD (depending on the cell type as antigen source) that is expressed on the lysosomal membrane of phagocytes as well as on the cell surface (weak expression). This antigen is the rat homologue of human CD68. The expression of this antigen in cells increases during phagocytic activity. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. This makes the monoclonal antibody ED1 a useful marker for rat macrophages. The monoclonal antibody ED1 is not able to block latex phagocytosis or bacterial killing.
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody ED1 can be used for Western blot and immunohistology on frozen and paraffin embedded sections. Furthermore the monoclonal antibody ED1 is useful for flow cytometry and immunoprecipitation. For paraffin sections protein digestion (e.g. trypsin or pronase) is required.
Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Damoiseaux, J et al; Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1. Immunology 1994, 83: 140
- Dijkstra, C et al; The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology 1985, 54:589
- Bauer, J et al; Phagocytic activity of macrophages and microglial cells during the course of acute and chronic relapsing experimental autoimmune encephalomyelitis. J Neurosci Res 1994, 38: 365
- Bao, F et al; Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats. J Neurochem 2004, 88: 1335
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available