TNF-alpha, Human, Recombinant (E. Coli-Derived) - HC2040

Name

TNF-alpha, Human, Recombinant (E. Coli-Derived)

Catalog nr

HC2040 (lot number and expiry date are indicated on the label)

Short description

Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative...

Size

5x10E4 units

Application

Technical datasheet

Description

The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines.

Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways.

The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.

One unit of Hycult Biotech Human Tumor Necrosis Factor alpha approximates the bioactivity of 16 units of the human Tumor Necrosis Factor alpha standard prepared by WHO as determined in L929 cytotoxic assay.

Formulation

0.5 ml of TNF-alpha with > 5*104 units in PBS. The exact unit definition is indicated on the label.

Species

Human, E.coli-derived

Use

For dilutions use a protein stabilized buffer pH 7.

Storage and stability

The stock solution should be stored in aliquots at -70°C. Avoid repeated freezing and thawing. Under recommended storage conditions, product stored at -70°C is stable for more than one year and at -20°C stable for 3 months.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available

HC1060	TNF-alpha, Mouse, Recombinant (E.Coli Derived)
HM2009	TNF-alpha, Human, mAb 4H31
HM2010	TNF-alpha, Human, mAb 52B83
HM2024	TNF-alpha, Human, mAb T1
HM2026	TNF-alpha, Human, mAb T3

MSDS

MSDS proteins and peptides.pdf

Related products		Cat #
TNF-alpha, Human, mAb T3		HM2026
TNF-alpha, Human, mAb T1		HM2024
TNF-alpha, Human, mAb 52B83		HM2010
TNF-alpha, Human, mAb 4H31		HM2009
TNF-alpha, Mouse, Recombinant (E.Coli Derived)		HC1060