Technical datasheet
Description
The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha.
TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.
Cross Reactivity
| Cross reactant |
Reactivity (Yes/No) |
| Rat TNF-RI |
Yes |
| TNF-RII |
No |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 1% bovine serum albumin and 0.02 % sodium azide.
Species
Human
Application
The biotinylated monoclonal antibody H398 can be used for immuno assay, flow cytometry, and Western blotting. Furthermore, the biotinylated monoclonal antibody H398 is useful for immunohistology on frozen sections. Be aware that the antibody competes with TNF-alpha.
Use
For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
CD120a, Tumor necrosis factor receptor superfamily member 1A, p55/p60, TNFR-1
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Thoma, B et al; Identification of a 60 kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J Exp Med 1990, 172: 1019
2. Grell, M et al; TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res 1993, 12: 143
3. Scheurich, P et al; Agonistic and antagonistic antibodies as a tool to study the functional role of human tumor necrosis factor receptors. Tumor Necrosis factor 1993, 4: 52
4. Grell, M et al; The type 1 receptor (CD120a) is the high-affinity receptor for soluble tumor necrosis factor. Proc Natl Acad Sci USA 1998, 95: 570
5. Krippner-Heidenreich, A et al; Single-chain TNF, a TNF derivative with enhanced stability and antitumoral activity. J Immunol 2008, 180: 8176
6. Kontermann, R et al; A humanized tumor necrosis factor receptor 1 (TNFR1)-specific antagonistic Antibody for selective inhibition of tumor necrosis factor (TNF) action. J Immunother 2008, 31: 225
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
References
1. Thoma, B et al; Identification of a 60 kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. J Exp Med 1990, 172: 1019
2. Grell, M et al; TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis. Lymphokine Cytokine Res 1993, 12: 143
3. Scheurich, P et al; Agonistic and antagonistic antibodies as a tool to study the functional role of human tumor necrosis factor receptors. Tumor Necrosis factor 1993, 4: 52
4. Grell, M et al; The type 1 receptor (CD120a) is the high-affinity receptor for soluble tumor necrosis factor. Proc Natl Acad Sci USA 1998, 95: 570
5. Krippner-Heidenreich, A et al; Single-chain TNF, a TNF derivative with enhanced stability and antitumoral activity. J Immunol 2008, 180: 8176
6. Kontermann, R et al; A humanized tumor necrosis factor receptor 1 (TNFR1)-specific antagonistic Antibody for selective inhibition of tumor necrosis factor (TNF) action. J Immunother 2008, 31: 225