Technical datasheet
Description
The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of soluble and membrane TNF-alpha. The antibody can be a useful tool to discriminate between receptor bound soluble (17 kDa) and the membrane (26 kDa) form of TNF-alpha. For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody HM2024, which recognizes only soluble and membrane TNF-alpha, but not the receptor bound TNF-alpha.
Formulation
0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The antibody is also useful for immuno precipitation, immuno assays, flow cytometry and Western blotting.
Use
For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
1. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
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HC2040
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TNF-alpha, Human, Recombinant (E. Coli-Derived)
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HM2009
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TNF-alpha, Human, mAb 4H31
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HM2010
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TNF-alpha, Human, mAb 52B83
|
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HM2024
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TNF-alpha, Human, mAb T1
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HM2026
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TNF-alpha, Human, mAb T3
|
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HP9001
|
TNF-alpha, Human, pAb
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References
1. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243