Technical datasheet
Description
The monoclonal antibody 5N reacts with Tumor Necrosis Factor alpha (TNF-alpha). Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms.
The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines.
Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways.
The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.
The monoclonal antibody 5N cross-reacts and cross-neutralizes all monkey species TNF-alpha, except pig-tail macaque. With affinity about two orders lower it recognizes bovine and canine TNF-alpha.
Cross Reactivity
| Cross reactant |
Reactivity |
| Bovine |
Yes |
| Canine |
Yes |
| Monkey |
Yes |
Formulation
0.5 mg (500 µg/ml) 0.2 µm filtered antibody solution in PBS (exact concentration is indicated on the label)
Species
Mouse IgG2a
Application
The monoclonal antibody 5N can be used for immuno assays. Furthermore the monoclonal antibody 5N is useful for the inhibition of biological activity of TNF-alpha.
Use
For neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. The affinity of the monoclonal antibody 5N is 5.7 x 10-10M. The homologous ELISA is useful for the detection of oligomeric human TNF-alpha. It does not recognize monomeric TNF-alpha.
Aliases
TNF, TNF-SF2, DIF, Cachectin
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Petyovka, N et al; Homologous ELISA for detection of oligomeric human TNF: properties of the assay. J Immunol Methods 1995, 186: 161
- Song, X et al; Effects of 2 different anti-tumor necrosis factor-alpha agents in a primate model of subcutaneous formation. J Infect Dis 2002, 185: 204
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Also available
|
HC2040
|
TNF-alpha, Human, Recombinant (E. Coli-Derived)
|
|
HM2010
|
TNF-alpha, Human, mAb 52B83
|
|
HM2026
|
TNF-alpha, Human, mAb T3
|
|
HM2005
|
TNF-RI, Human, mAb MR1-2
|
|
HM2007
|
TNF-RII, Human, mAb MR2-1
|