Technical datasheet
Description
The polyclonal antibody recognizes the extracellular part of the mouse Tumor Necrosis Factor Receptor type 2 (TNF-RII) of the membrane-bound as well as the soluble receptor. TNF-RII (~75-80 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. Binding of the inherently trimeric TNF-alpha to TNFR1 and TNFR2 induces receptor trimerization and recruitment of several signaling proteins to the cytoplasmic domains of the receptors. Occupancy of TNFR2 results in direct recruitment of TNF Receptor Associated Factor 2 (TRAF2), which in turn recruits TRAF1.
Cross Reactivity
Cross reactant | Reactivity |
Mouse TNF-RI Mouse TNF-alpha | Yes* Minimal crossreactivity |
* problematic in case of TNF-RII knockout studies and when used in high concentrations in functional studies |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin
Species
Rabbit IgG
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | | ● | ● | ● | | ● | | ● | | No | | | | | | | | | | N.D. | ● | | | | ● | | ● | | |
N.D.=Not Determined; F=Frozen sections; FC=Flow cytometry; FS=Functional studies; IA=Immuno assays; IF=Immuno fluorescence; IP=Immuno precipitation; P=Paraffin sections; W=Western blot |
Use
For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Aliases
Tumor necrosis factor receptor superfamily member 1B, TNF-RII, p75/p80
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Lucas, R et al; Both TNF receptors are required for direct TNF-mediated cytotoxicity in microvascular endothelial cells. Eur J Immunol 1998, 28: 3577
- Bemelmans, M et al; LPS-Induced sTNF-receptor release in vivo in a murine model. J Immunol 1993, 151: 5554
- Gerspach, J et al; Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents. Micr Res Tech 2000, 50: 243
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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