Technical datasheet
Description
Tumor Necrosis Factor alpha (TNF-alpha) is a cytokine which has diverse immunomodulatory, anti?tumor and toxic effects. TNF-alpha has been detected in diverse inflammatory status and appears to be a critical mediator in the lethality of septic shock. Furthermore, TNF-alpha has also been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. The antibody reacts with both rat and mouse natural and recombinant TNF-alpha and recognizes membrane and receptor bound TNF-alpha. The antibody shows neutralizing activity.
Cross Reactivity
| Cross reactant |
Reactivity |
| Rat |
Yes |
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Rabbit IgG
Application
The antibody can be used for inhibition of biological activity. Furthermore the antibody is useful for immuno histology on both frozen and paraffin sections, flow cytometry, immuno assay and Western blotting.
Use
For immuno histology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts TNF-alpha to be inactivated.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
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