Name
sTNF-RII, Human, ELISA kit
Catalog nr
HK302
(lot number and expiry date are indicated on the label)
Short description
Tumor Necrosis Factor is a pleiotropic inflammatory cytokine. On almost all cells receptors for TNF have been detected. TNF-Receptors (TNF-R) were originally designated as TNF-binding proteins. These TNF-binding proteins present in urine and plasma were later found to be the extra-cellular part of the membrane bound cell signalling receptor for TNF-alpha and TNF-beta. Two types of TNF-Recepto...
Size
2 x 96 det.
Application
Manual
Description
Tumor Necrosis Factor is a pleiotropic inflammatory cytokine. On almost all cells receptors for TNF have been detected. TNF-Receptors (TNF-R) were originally designated as TNF-binding proteins. These TNF-binding proteins present in urine and plasma were later found to be the extra-cellular part of the membrane bound cell signalling receptor for TNF-alpha and TNF-beta. Two types of TNF-Receptors (molecules belonging to the family of nerve growth receptor, CD27, CD40 etc) have been identified: TNF-RI (p55/p60), sCD120a and TNF-RII (p75/p80), sCD120b. The molecules share substantial identity in their extracellular part although the intra-cellular parts are structurally unrelated. This difference is reflected by the difference in function of the two receptors.
Activation of various cell types leads to proteolytic cleavage of TNF-R resulting in soluble (extracellular part) TNF-R. Soluble TNF-R (sTNF-R) are considered to play a physiological role in TNF binding and inactivation, although also other functions such as stabilization of TNF in circulation have been mentioned. Detection of sTNF-R offers a means of investigating activation of the immune system both in vitro and in vivo. In plasma of healthy individuals approx. 1 - 4 ng/ml of sTNF-R of each type are present.
The ELISA kit is also useful for quantitation of Rhesus monkey and Baboon sTNF-RII in plasma, serum and tissue culture media.
Application
The sTNF-RII ELISA has been developed for the quantitative measurement of natural human sTNF-RII (sCD120b) in serum, plasma and culture medium. In serum or plasma samples sTNF-RII can be measured accurately if serum or plasma samples are diluted at least 10 times. Most reliable results are obtained if EDTA plasma is used.
Features
- Minimum concentration which can be measured is 25 pg/ml sTNF-RII
- Measurable concentration range of 15.6-1,000 pg/ml.
- Working volume of 100 µl/well.
Typical standard curve
Principle
- The sTNF-RII ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4½ hours.
- The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay.
- Samples and standards are captured by a solid bound specific antibody.
- Biotinylated tracer antibody will bind to captured sTNF-RII.
- Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody.
- Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB).
- The enzyme reaction is stopped by the addition of citric acid.
- The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the sTNF-RII standards (log).
- The sTNF-RII concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least six months. After reconstitution the reagents are stable for 1 month if stored at 2-8°C.
References
- Aziz, N et al; Stability of plasma levels of cytokines and soluble activation markers in patients with human immunodeficiency virus infection. J Infect Dis 1999, 179: 843
- Maier, B et al; Delayed elevation of soluble tumor necrosis factor receptors p75 and p55 in cerebrospinal fluid and plasma after traumatic brain injury. Shock 2006, 26: 122
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
References
- Aziz, N et al; Stability of plasma levels of cytokines and soluble activation markers in patients with human immunodeficiency virus infection. J Infect Dis 1999, 179: 843
- Maier, B et al; Delayed elevation of soluble tumor necrosis factor receptors p75 and p55 in cerebrospinal fluid and plasma after traumatic brain injury. Shock 2006, 26: 122
MSDS
| Related products | Cat # | |
|---|---|---|
| TNF-alpha, Human, ELISA kit | HK307 | |
| sTNF-RI and sTNF-RII, Human, Twin ELISA kit | HK303 | |
| sTNF-RI, Human, ELISA kit | HK301 | |