TLR2, Human, mAb TL2.3, biotinylated - HM2067

Innate Immunity

Inflammation

Cell- and tissue damage

Name

TLR2, Human, mAb TL2.3, biotinylated

Catalog nr

HM2067 (lot number and expiry date are indicated on the label)

Short description

Size

50 µg

Application

FC , IA , W

Technical datasheet

HM2067.pdf

Description

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282).

The TL2.3 monoclonal antibody is specific for human TLR2 (CD282). TL2.3 is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human peripheral blood mononuclear cells.

The monoclonal antibody TL23 is cross reactive with canine TLR2.

Formulation

0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

Species

Mouse IgG2a

Application

The antibody can be used for flow cytometry, Western blotting and immuno assays.

Use

For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Storage and stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

References

1. Lien, E et al; Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem 1999, 274: 33419

2. Flo, TH et al; Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 2001, 69: 474

3. Lien, E et al; A novel synthetic acyclic lipid A-like agonist activates cells via the lipopolysaccharide/toll-like receptor 4 signaling pathway. Biol Chem 2001, 276: 1873

4. Flo, TH et al; Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol 2000, 164: 2064

5. Faure, E et al; Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol 2001, 166: 2018

6. Wang, JE et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001, 69: 2402

7. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. The human Toll-like receptor 2 (TLR2) is Patent protected by US Patent no. US 7,071,310. License rights on the Patent are acquired from Leiv Eriksson Nukaping AS, Norway. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Also available

HM2064	TLR2, Human, mAb TL2.1
HM2065	TLR2, Human, mAb TL2.1, biotinylated
HM2066	TLR2, Human, mAb TL2.3
HM2068	TLR4, Human, mAb HTA125
HM2085	TLR1, Human, mAb GD2.F4

References

1. Lien, E et al; Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem 1999, 274: 33419

2. Flo, TH et al; Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 2001, 69: 474

3. Lien, E et al; A novel synthetic acyclic lipid A-like agonist activates cells via the lipopolysaccharide/toll-like receptor 4 signaling pathway. Biol Chem 2001, 276: 1873

4. Flo, TH et al; Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol 2000, 164: 2064

6. Wang, JE et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001, 69: 2402

7. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184

MSDS

MSDS biotinylated antibodies with azide.pdf

Protocols

Protocol Flow cytometry.pdf

Protocols

Protocol Sandwich ELISA.pdf

Protocols

Protocol Western Blotting.pdf

Related products		Cat #
TLR4, Human, mAb HTA125		HM2068
TLR2, Human, mAb TL2.1		HM2064
TLR2, Human, mAb TL2.3		HM2066
TLR2, Human, mAb TL2.1, biotinylated		HM2065
TLR1, Human, mAb GD2.F4		HM2085