Technical datasheet
Description
The monoclonal antibody 19D759.2 recognizes an intracellular epitope in the cytoplasmic domain of human TLR5. TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities. The various TLRs exhibit different patterns of expression.
TLR5 is upregulated in response to exposure to flagellated bacteria or the TLR5 agonist, flagellin. Gram-negative bacteria stimulate monocyte/macrophage cells in a TLR5 specific, CD14-independent manner. The innate immune system recognizes flagellated bacterial pathogens through the TLR5 receptor signalling. It acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1).
Cross Reactivity
Cross reactant | Reactivity |
Mouse Dog | Yes Yes |
Immunogen
Synthetic peptide corresponding to a portion of human TLR5 between amino acid 700-800 conjugated to KLH
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG2a
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | ● | ● | | | ● | | ● | ● | | No | | | | | | | | | | N.D. | | | ● | ● | | ● | | | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For immunohistology, immunofluorescence, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Toll-like receptor 5, Toll Interleukin-1 receptor-like protein 3
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Miller, L et al; TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol 2005, 174: 6137
- Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124: 184
- Deng, J et al; Toll-like receptors 4 and 5 induce distinct types of vasculitis. Circ Res 2009, 104: 488
- Ritter, M et al; Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adapter proteins and inflammatory response. J of Inflammation 2005, 2: 16
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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