Technical datasheet
Description
The monoclonal antibody 3C3 reacts with human Toll-like receptor 4 (TLR4, CD284). TLRs belong to a family of proteins that specifically recognize and sense microbial products. They are highly conserved throughout evolution. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. TLRs identified as type I transmembrane signalling receptors act as innate immune recognition receptors against many pathogens.
TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria.
The monoclonal antibody 3C3 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells.
Immunogen
CHO cells expressing chimeric human TLR4/MD-2 fusion protein
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Species
Mouse IgG1
Application
The monoclonal antibody 3C3 can be used for flow cytometry. Furthermore, the antibody can be used for functional studies as antagonist for TLR4. It can be used by blocking LPS-induced cytokine functions.
Use
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Also available
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HM2247BT
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TLR4, Human, mAb 3C3, biotinylated
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HM2247F
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TLR4, Human, mAb 3C3, FITC
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HM2243
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sMD-2 and sMD-2/TLR4, Human, mAb 4H1
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HM2245
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MD-2, Human, mAb 18H10
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HM2246
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TLR4/MD-2, Human, mAb 7E3
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