Technical datasheet
Description
Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In
Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that is central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma,
Staphylococcus aureus, and
Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282).
The monoclonal antibody TL2.3 is specific for human TLR2 (CD282). TL2.3 is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human peripheral blood mononuclear cells.
The monoclonal antibody TL23 is cross reactive with canine TLR2.
Immunogen
Human TLR2-expressing CHO cells
Formulation
100 tests 0.2 µm filtered antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Species
Mouse IgG2a
Application
The monoclonal antibody TL2.3 can be used for Western blotting, immuno assay and flow cytometry. Furthermore, the monoclonal antibody TL2.3 is useful for immunofluorescence, immunohistology on frozen sections and functional studies. The monoclonal antibody TL2.3 can be used for stimulation of T cells..
Use
For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Aliases
CD282, TIL4
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Flo, T et al; Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 2001, 69: 474
- Schjetne, K et al; Link between innate and adaptive immunity: Toll-like receptor 2 internalizes antigen for presentation to CD4+ T cells and could be an efficient vaccine target. J Immunol 2003, 171: 32
- Siedlar, M et al; Tolerance induced by the lipopeptide Pam3Cys is due to ablation of IL-1R-associated kinase 1. J Immunol 2004, 173: 2736
- Tunheim, G et al; Human receptors of innate immunity (CD14, TLR2) are promising targets for novel recombinant immunoglobulin-based vaccine candidates. Vaccine 2007, 25: 4723
- Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. The human Toll-like receptor 2 (TLR2) is Patent protected by US Patent no. US 7,071,310. License rights on the Patent are acquired from Leiv Eriksson Nukaping AS, Norway. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
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