Technical datasheet
Description
The polyclonal antibody recognizes human Toll-like receptor 7. TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. Like its counterparts in Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR are identified as type I transmembrane signaling receptors with pattern recognition capabilities. The various TLRs exhibit different patterns of expression. The
TLR7 gene is predominantly expressed in lung, placenta, and spleen, and is located in close proximity to another family member,
TLR8, on human chromosome X.
TLR3, 7, 8 and 9 belong to a third class of TLRs which are localized intracellular where they detect nucleic acids derived from viruses and bacteria. TLR7 is recognized by single stranded RNA in endosomes, which is a common feature of viral genomes which are internalized by macrophages.
TLR7 proteins are localized in endosomes and/or lysosomes. Recently, it has been discovered that TLR3, TLR7 and TLR9 trafficking requires UNC93B1, a 12-membrane spanning protein that resides in the endoplasmatic reticulum. Mice or cells that lack UNC93B1 function, are defective in TLR3, TLR7 and TLR9 signaling. Association of UNC93B1 with TLRs in the ER facilitates their transport to the endolysosome.
NK cells are activated through TLR7/8 and report a central role for monocyte-derived IL-12 in the differential IFN- produced by NK cells in response to different TLR agonists.
Cross Reactivity
Cross reactant | Reactivity |
Mouse Rat | Yes Yes |
Immunogen
Synthetic peptide of human TLR7 corresponding to amino acids 706-728 conjugated to KLH
Formulation
1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rabbit IgG
Application
| | F | FC | FS | IA | IF | IP | P | W | | Yes | ● | ● | | | ● | ● | | ● | | No | | | | | | | | | | N.D. | | | ● | ● | | | ● | | |
N.D.= Not Determined; F = Frozen sections; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IF = Immuno Fluorescence; IP = Immuno Precipitation; P = Paraffin sections; W = Western blot |
Use
For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Aliases
Toll-like receptor 7
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- François, S et al; Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-{kappa}B signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated bad. J Immunol 2005, 174: 3633
- Patole, P et al; Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice. Nephrol Dial Transplant 2006, 21: 3062
- Pawar, R et al; Toll-like receptor-7 modulates immune complex glomerulonephritis. J Am Soc Nephrol 2006, 17: 141
- Brinkmann, M et al; The interaction between the ER membrane protein UNC93B and TLR3, 7 and 9 is crucial for TLR signaling. J Cell Biol 2007, 177: 265
- Clark, R et al; Human squamous cell carcinomas evade the immune response by down- regulation of vascular E-selectin and recruitment of regulatory T cells. J Exp Med 2008, 205: 2221
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
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