Technical datasheet
Description
The monoclonal antibody MD113 reacts with mouse MD-1. MD-1 (22-25 kD) is an extracellular protein associated with the extracellular domain of RP105. The latter is a type 1 transmembrane protein with leucine-rich repeats. RP105 is similar to Drosophila Toll. MD-1 associated with RP105 (similar to TLR4/MD-2) appears to be an essential molecule that helps RP105 on B-cells to signal to LPS. The MD113 antibody is antagonistic in LPS-induced B-cell proliferation and B7.2 up-regulation.
Formulation
1 ml (100 µg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Species
Rat IgG2b
Application
The monoclonal antibody MD113 can be used for flow cytometry and functional studies. The monoclonal antibody cannot be used for Western blot and immunoprecipitation.
Use
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
References
- Miyake, K et al; Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 1998, 161: 1348
- Nagai, Y et al; Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood 2002, 99: 1699
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Also available
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HM1040F
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MD-1, Mouse, mAb MD113, FITC
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HM1041
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MD-1, Mouse, mAb MD113, biotinylated
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HM1031
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CD180, Mouse, mAb RP/14
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HM1032
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CD180, Mouse, mAb RP/14, biotinylated
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HM1029
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TLR4/MD-2, Mouse, mAb MTS510
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