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Short summary of Zhang, X et al; Low levels of ficolin-3 are associated with diabetic peripheral neuropathy. Acta Diabetol 2016. More information about the article can be found here.

 

Diabetic peripheral neuropathy (DPN) is one of the most common chronic complications of diabetes. Although a wide range of screening and diagnostic tools […] are available, there still is a lack of convenient and sensitive biomarkers. This study investigates the relation between serum ficolin-3 levels and DPN in patients with diabetes mellitus.

 

The study was performed with a total of 466 diabetes patients. They were tested for a variety of metabolic markers and also neuropathy was assessed. On top of this, Ficolin-3 was tested (Cat. # HK340) and compared with the diagnostic criteria that were set in this study.

 

Looking at the results, it was clear that the serum level of ficolin-3 in the DPN group was significantly lower than the non-DPN group. The data not only showed an implication for the DPN group, also patients with only diabetic nephropathy or only diabetic retinopathy all had significantly lower ficolin-3 levels. DPN prevalence also significantly depended on the ficolin-3 serum concentrations. Based on these results, ficolin-3 demonstrates to be an independent impact factor on the risk of DPN next to age and diabetes duration. Further studies revealing the immanent connection of ficolin-3 with the pathology of diabetic peripheral nerve disorders will provide a new prospective strategy for DPN.


Posted in Scientific Publications By G. Snippe

Short summary of Huang, Z et al; Both systemic and local lipopolysaccharide (LPS) burden are associated with knee OA severity and inflammation. Osteoarthritis Cartilage 2016, 24: 1769. Full article can be found here 

Osteoarthritis (OA) is a heterogeneous disease with differing predisposing conditions including aging, trauma, genetics and obesity. LPS is known to contribute to low-grade inflammation with a number of clinical conditions, including cardio-metabolic dysfunction, acceleration of atherosclerosis, and diabetes. LBP is involved in the macrophage response to LPS. This study investigates the role of LPS in the pathogenesis of OA and explain the link between obesity and OA. For this purpose, both LPS and LBP were measured in serum and synovial fluid.

 The results demonstrated that both LPS and LBP were significantly associated with activated macrophages in the knee, radiographic OA-severity and joint symptoms.

These data support a role of LPS in the pathogenesis of OA through the activation of macrophages. Strategies for lowering LPS could be important treatments for OA in a subset of individuals with measurable concentrations.

Posted in Scientific Publications By G. Snippe

Short summary of Matusiak, A et al; Putative consequences of exposure to Helicobacter pylori infection in patients with coronary heart disease in terms of humoral immune response and inflammation. Arch Med Sci 2016, 12(1). Full article can be found here.

Pathogens have been suggested to contribute to the development of coronary heart disease (CHD). So far there was no consensus on the role of Helicobacter pylori (Hp) infections in either causation or progression of CHD. Recently it has been shown that acute phase proteins can function as pattern recognition receptors (PRR) and are therefore potentially involved in the pathogenesis of cardiovascular disease. To create a more thorough understanding of this process, this study focuses on the exposure of CHD patients to Hp in relation to the level of serum lipopolysaccharide binding protein (LBP) as a marker of inflammation.

170 CHD patients and 58 non-CHD subjects participated in this study. All individuals were tested for H. pylori and LBP levels were also determined. The results demonstrated that CHD patients were exposed to Hp more frequently compared to the control group. The study also showed that the LBP levels were significantly higher in CHD patients and it was also related to the severity of the disease. Additionally, type I Hp strains stimulated higher LBP levels than the less pathogenic type II.

The results generated in this study support the hypothesis of a relationship between Hp infections and CHD. It also underlines the fact that high levels of LBP accelerate inflammatory processes that contribute to disease severity.

 

Posted in Scientific Publications By G. Snippe

Short summary of Tamaki, K et al; Fibrocytes and fibrovascular membrane formation in proliferative diabetic retinopathy. Invest Ophthalmol Vis Sci 2016, 57(11). Full article can be found here.

Diabetic retinopathy is classified in four stages of which proliferative diabetic retinopathy (PDR) is the most advanced stage. It is characterized by the formation of a fibrovascular membrane (FVM), which is the result of chronic inflammation and ischemia.

A circulating bone marrow-derived population of fibroblast-like cells, termed fibrocytes, has recently been suggested to have a role in the pathogenesis of fibrosis in various organs. Serum amyloid P component (SAP) is a highly conserved protein and is a soluble pattern recognition receptor involved in the innate immune system that regulates monocyte activation and differentiation. SAP inhibits the differentiation of circulating monocytes into fibrocytes. There have been several reports asserting that the circulating level of SAP may have an important role in fibrosis.

The SAP concentration was significantly higher in vitreous fluid samples from PDR patients than in those from control samples (77.5 ± 53.3 ng/ml versus 1.4. ± 1.1 ng/ml, P < 0.05). These results suggest that the pathogenesis of FVM may be related to infiltration of fibrocytes into the vitreous fluid, which may be regulated by SAP. Accordingly, fibrocytes may be a novel therapeutic target for treatment of PDR.

Posted in Scientific Publications By G. Snippe

Short summary of Uhde, M et al; Intestinal cell damage and systemic immune activation in individuals reporting sensitivity to wheat in the absence of coeliac disease. Full article can be found here.

Celiac disease is characterized by an immune response in the small intestine when wheat gluten are consumed. However, there remains a group of individuals that experience a range  of symptoms in response to ingestion of wheat and related cereals, […] while celiac disease and IgE-mediated wheat allergy have been ruled out. Non-celiac wheat sensitivity (NCWS) is the term that is generally used to describe this condition. This paper aims to determine if sensitivity to wheat in the absence of celiac disease is associated with systemic immune activation that may be linked to an enteropathy. LBP and EndoCab® were some of the serological markers that were used to determine immune response to microbial components.

 

The results demonstrated that individuals with NCWS had significantly increased serum levels of LBP and Endocab® IgM, but not IgG or IgA. This while the celiac disease group had higher levels of EndoCab® IgA.

Even though the increased serum levels of the different markers demonstrate an underlying immunological response, this study does not address the potential mechanism or molecular triggers responsible for deteriorated gut integrity and microbial translocation.

Posted in Scientific Publications By G. Snippe

Short summary of Lima, C et al; Therapeutic Effects of Treatment with Anti-TLR2 and Anti-TLR4 Monoclonal Antibodies in Polymicrobial Sepsis. Full article can be found here.

 

Toll-like receptors (TLRs) play an important role in the recognition of exogenous and endogenous danger signals. Although signaling through TLRs is an important element of host defense, a growing body of evidence indicates that dysregulation of these receptors may also play a role in the pathogenesis of sepsis. The massive release of inflammatory mediators into the bloodstream following TLR activation is suspected to be associated with sepsis, culminating in multiple organ failure.

 

Sepsis-induced mice were administered with antibodies blocking TLR2 (mAb T2.5 HM1054-FS) or TLR4 45 minutes before or 3 hours after sepsis induction. The results demonstrate that blockade of TLR2 or TLR4 was successful in decreasing disease severity in sepsis models of Gram-negative and-positive bacteria. This outcome reinforces the role of TLRs in the pathogenesis of sepsis and suggest that blockade of TLR2 or TLR4 may be a useful therapeutic approach to control systemic inflammation, organ injury and lethality in polymicrobial sepsis.


Posted in Scientific Publications By G. Snippe

Hycult Biotech participates in new H2020 project aiming at improved diagnostics and intervention strategies for chronic autoimmune diseases.

 

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Posted in Hycult Biotech Press Releases By K. Peeters

Short summary of Vivekanandhan Aravindhan et al; Chronic Endotoxemia in Subjects with Type-1 Diabetes Is Seen Much before the Onset of Microvascular Complications

Lipopolysaccharide (LPS)/Endotoxin is hypothesized to play an important role in chronic inflammation associated with Type-1 diabetes (T1DM) and its complications. Endotoxin core antibodies (EndoCAb), LPS binding protein (LBP) and soluble CD14 (sCD14) act as modulators of LPS induced activation of innate immune system in vivo. Compared to normal glucose tolerance (NGT), T1DM subjects (both with and without microvascular complications (MVC)) had significantly higher levels of LPS, reduced levels of LBP and EndoCAb along with significant increase in the levels of IL-1β, IL-6, TNF-α and GM-CSF (p<0.05). No significant change was seen in the levels of these biomarkers between T1DM subjects with and without MVC.

Decreased levels of EndoCAb and LBP suggest sustained endotoxin activity in T1DM subjects even before the onset of microvascular complications.

HIT302

 

Serum levels of LPS (a), LBP (b), sCD14 (c) and EndoCAb (d) were determined in NGT (n = 64), T1DM without MVC (n = 97) and T1DM subjects with MVC (n = 36) by immune-assays. Each dot represents individual values with the horizontal line representing the geo mean. 36 T1DM subjects without MVC and 26 with MVC had undetectable levels of EndoCAb.


Posted in Scientific Publications By K. Peeters

KYC is a reversible inhibitor of MPO and does not irreversibly damage the enzyme as compared to other substrate inhibitors.

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Posted in Scientific Publications By K. Peeters

Two new Calprotectin ELISA's have been released for quantitative determination of Calprotectin: HK382 & HK379.

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Posted in Product News By K. Peeters

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