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Tsalkidou, E.A et al. Pediatr. Nephrol. 2013 March 6.

Children frequently suffer from urinary tract infections. Current clinical practice is evaluation of conventional serum markers like total white blood cell count, erythrocyte sedimentation rate, C-reactive protein (CRP), procalcitonin (PCT) and Interleukin-6 (IL-6).

Accurate diagnosis and early treatment of urinary tract infections are of pivotal importance in particularly for infancy and early childhood. A candidate marker is LBP, an acute phase protein produced by the liver. LBP has high affinity to lipopolysaccharide (LPS) and in transferring monomeric LPS to CD14. CD14 accelerate LPS recognition by Toll Like Receptor 4 (TLR-4) which induces the release of cascade of pro-inflammatory cytokines. These cytokines are the key mediators in the pathogenesis of the systemic inflammatory response.

LBP is present in plasma of healthy individuals at concentrations of 2 – 10 mg/l and rapidly increases in case of infections. This study reports that inflammation–free children at the age of 10 years have a mean concentration of 27.66 mg/l and a range of 11.82 -43.50 mg/l. Children with urinary tract infections have a mean concentration of 95.55mg/l and range of 36.75 – 241.25 mg/l.

The results show a sensitivity, negative predictive value, specificity and positive predictive value superior for LBP. These findings suggest that LBP is a better diagnostic marker for urinary tract infections then currently used diagnostic markers.

Products:

 

LBP, Human, ELISA kit  2 x 96 det.   Cat.# HK315-02 
LBP, Human, ELISA kit  1 x 96 det.  Cat.#  HK315-01 
LBP, Mouse, ELISA kit  1 x 96 det.   Cat.# HK205-01 
LBP, Mouse, ELISA kit  2 x 96 det.    Cat.# HK205-02 
sCD14, Human, ELISA kit  1 x 96 det.   Cat.# HK320-01 
sCD14, Human, ELISA kit 2 x 96 det.  Cat.# HK320-02 
LBP, various species, ELISA kit 1 x 96 det.  Cat.#  HK503   
EndoClear, red, small (EndoTrap red 1/1 + mini LAL)     Cat.# HIT306 
EndoClear, blue, small (EndoTrap blue 1/1 + mini LAL)     Cat.# HIT305 
IL-FABP, Human, pAb 100 µg  IA W  Cat.#HP9031   
A-FABP, Human, pAb 100 µg IA W  Cat.# HP9028  
LBP, Human, pAb 100 µg IA IP W  Cat.#HP9023 
IL-FABP, Mouse, pAb 100 µg  IA P W  Cat.#HP8011  
Lipopolysaccharide Core, mAb WN1 222-5 100 µg  FS IA P W  Cat.#HM6011
Lipopolysaccharide (LPS) from E.coli, Serotype O8:K27 1 mg  FS  Cat.# HC4065  
TLR4 agonist Set: Lipopolysaccharide (LPS) from E.coli  FS  Cat.# HC4064 

 

Posted in Scientific Publications Product News By Kim Peeters

LPS-core mAb, clone WN222-5

Feb 20, 2014 1:45:06 AM

Now available: Lipopolysaccharide Core, mAb WN1 222-5, cat# HM6011 previously known as HM6001.


 Images kindly provided by Dr. Jacob D. Estes, AIDS & Cancer Virus Program, SAIC-Frederick Inc., Frederick National Laboratory for Cancer Research LPS staining within the colon lumen of SIV-infected rhesus macaques

Images kindly provided by Dr. Jacob D. Estes, AIDS & Cancer Virus Program, SAIC-Frederick Inc., Frederick National Laboratory for Cancer Research

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Posted in Product News By Kim Peeters

Change of names Ficolin products

Nov 5, 2013 3:05:48 AM

We have decided to change the names of our ficolin products...

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Posted in Product News By Kim Peeters

M-ficolin (ficolin-1) is a complement-activating pattern-recognition molecule structurally related to mannan-binding lectin. It is produced by monocytes and neutrophils, and is found in serum...

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Posted in Scientific Publications Product News By Kim Peeters

CFH Y402H genotype and CD56+ T cells in age-related macular degeneration

Age-related macular degeneration (AMD), the commonest cause of blindness in the developed world, is a late-onset disease. More than 10% of people aged 60 years or older are affected and the prevalence increases exponentially with age, reflecting that age is the strongest risk factor for AMD. The Y402H single nucleotide polymorphism (SNP) in complement factor H (CFHY402H) represents another great risk factor. Further, circumstantial evidence has suggested a role of T cells in the immunopathogenesis of AMD.

Based on the hypothesis that age-related changes of the T cell compartment contribute to the development of AMD, we tested whether CD56+ T cells, known to increase with age, were associated with AMD. We found increased levels of CD56+ CD28- T cells in patients with both early and late forms of AMD. Although the frequency of CD56+ T cells was partly dependent on CFHY402H genotype, the adjusted odds ratio for the prevalence of AMD of the two factors combined was markedly increased compared to the odds ratio based on CFHY402H genotype alone. Together, these results associate systemic cellular changes in the adaptive immune system with AMD, which supports the understanding of AMD as a systemic disease.

Ref: Age-Related Macular Degeneration Is Associated with Increased Proportion of CD56+ T Cells in Peripheral Blood. Faber C, Singh A, Krüger Falk M, Juel HB, Sørensen TL, Nissen MH. Ophthalmology. 2013 Jun 6. doi:pii: S0161-6420(13)00361-8. 10.1016/j.ophtha.2013.04.014. [Epub ahead of print]

Table

Odds ratios for prevalence of age-related macular degeneration according to frequency of CD56+ CD28- T cells and CFHY402H genotype.

 

 

Related products:

Factor H, 402H/Y variant detection, Human, ELISA kit, cat# HK353

Complement Factor H, Human, ELISA kit cat# HK342-01 and HK342-02

Complement Factor H, Human CE IVD ELISA kit cat# DH003

 

Posted in Scientific Publications Product News By Kim Peeters

CC16 and SP-D plasma levels are associated with clinical outcomes of mechanically ventilated patients.

Major causes of morbidity and mortality in Intensive Care Unit patients are acute lung injury (ALI) and the more severe form acute respiratory distress syndrome (ARDS). Improving the identification of patients with ALI/ARDS allows clinicians to practice specific procedures.

Biological markers are potential diagnostic tools to use. Previous studies have shown that Clara Cell Protein (CC16) and Surfactant Protein D (SP-D) are diagnostic plasma markers of ALI/ARDS in patients with ventilator-associated pneumonia.

In the present study, the relationship was examined between plasma levels of CC16 and SP-D and the clinical scores. The results revealed that plasma levels of CC16 correlate well with the lung injury score, oxygenation index and SOFA score at days 0, 2 and 4. For SP-D the same correlations were observed but now only after 4 days of mechanical ventilation.

Furthermore, development of lung injury was associated with a 3 fold increase in plasma SP-D levels while CC16 levels were only moderately increased in patients with lung injury.

CC16 is a protein that is produced by Clara cells in the small airways. SP-D is a protein produced by alveolar type II cells and Clara cells. Both proteins are associated with leakiness of the respiratory membrane. Because CC16 is a small protein the leakage cannot be related with the lung injury. Increased leakage of the larger protein SP-D in the blood is highly indicative of more damage in the lung. However, increased SP-D levels were only observed after a couple of days when the lung injury was at a certain rate.

More studies need to be performed to determine the diagnostic accuracy of these markers.

Reference

Determann, RM et al. Clara Cell Protein and Surfactant Protein D plasma levels are associated with clinical outcomes of mechanically ventilated patients. J Pulmon. Resp. Med. 2013, 3:1

Products

SP-D, Human, ELISA kit 2 x 96 det. HK335-02  
SP-D, Human, ELISA kit 1 x 96 det. HK335-01  
SP-D, Rat, mAb IIIH3  100 µg IA P W HM3022
SP-D, Rat, mAb VIF9  100 µg IA P W HM3023
SP-D, Rat, mAb IVG8  100 µg IA P W HM3024
SP-B, Pig, pAb  100 µg IA W HP7002
SP-B, Human, pAb  100 µg F P W HP9049
Pro SP-C, Human, pAb  100 µg F P W HP9050
Posted in Scientific Publications Product News By Kim Peeters

Plasma biomarkers of pulmonary and extra-pulmonary tuberculosis in children

Kumar, NP et al. Circulating biomarkers of pulmonary and extra-pulmonary tuberculosis in children. Clin. Vaccine Immunol. 2013, 13 March.

The global impact of childhood tuberculosis is unknown because of lack of reports. Under adults there is an estimated 9 million new infections and 1.7 million deaths per year. It is generally reported that at least 10-15% of cases in the world occur in children.

The golden standard for diagnosing TB is microbiological culture, however this often fails in children. Recent studies also show that tests based on nucleic acid amplification fail with the same reasons. Immuno assays based on plasma samples can possibly improve the diagnosis.

In this study, many biomarkers of the pathogenesis of TB have been examined. Also a few markers of the innate immunity have been examined, including LPS, LBP, sCD14 and antibodies to the LPS core (EndoCab). In previous papers, these markers have proven their role in various infectious diseases like HIV, hepatitis and parasitic infections.

The current findings reveal that LPS, LBP and EndoCab levels can serve  as discriminating markers between pulmonary and extra-pulmonary disease in children. Suppressed levels of LPS and LBP and increased levels of EndoCab are present in extra-pulmonary disease compared to pulmonary disease. Interestingly, the levels between healthy control children and children with pulmonary disease were not significant different. This means that persistant immune activation is not need fully a hallmark of pulmonary TB in children.

In conclusion, this study reports the potential of certain factors in diagnosing pulmonary and extra-pulmonary disease in children. More studies are needed to provide more insight in pediatric tuberculosis.

 

Products    
LAL Chromogenic Endpoint Assay  3 x 96 det.   HIT302 
EndoCab IgM, Human, ELISA  1 x 96 det.   HK504-IgM   
EndoCab IgG, Human, ELISA  1 x 96 det.   HK504-IgG 
EndoCab IgA, Human, ELISA  1 x 96 det.   HK504-IgA 
EndoCab, Human, ELISA kit  1 x 96 det.   HK504
LBP, Human, ELISA kit  1 x 96 det.   HK315-01 
TNF-alpha, Human, ELISA kit  1 x 96 det.   HK307-01   
IFN-gamma, Human, ELISA kit (medium & serum)  1 x 96 det.   HK309-01 
SAA, Human, ELISA kit  1 x 96 det.   HK333-01 
CRP, Human, ELISA kit  1 x 96 det.   HK358     
sCD14, Human, ELISA kit  1 x 96 det.   HK320-01

 

Posted in Scientific Publications Product News By Kim Peeters

Tsalkidou, E.A et al. Pediatr. Nephrol. 2013 March 6.

Children frequently suffer from urinary tract infections. Current clinical practice is evaluation of conventional serum markers like total white blood cell count, erythrocyte sedimentation rate, C-reactive protein (CRP), procalcitonin (PCT) and Interleukin-6 (IL-6).

Accurate diagnosis and early treatment of urinary tract infections are of pivotal importance in particularly for infancy and early childhood. A candidate marker is LBP, an acute phase protein produced by the liver. LBP has high affinity to lipopolysaccharide (LPS) and in transferring monomeric LPS to CD14. CD14 accelerate LPS recognition by Toll Like Receptor 4 (TLR-4) which induces the release of cascade of pro-inflammatory cytokines. These cytokines are the key mediators in the pathogenesis of the systemic inflammatory response.

LBP is present in plasma of healthy individuals at concentrations of 2 – 10 mg/l and rapidly increases in case of infections. This study reports that inflammation–free children at the age of 10 years have a mean concentration of 27.66 mg/l and a range of 11.82 -43.50 mg/l. Children with urinary tract infections have a mean concentration of 95.55mg/l and range of 36.75 – 241.25 mg/l.

The results show a sensitivity, negative predictive value, specificity and positive predictive value superior for LBP. These findings suggest that LBP is a better diagnostic marker for urinary tract infections then currently used diagnostic markers.

Products:

 

LBP, Human, ELISA kit  2 x 96 det.   Cat.# HK315-02 
LBP, Human, ELISA kit  1 x 96 det.  Cat.#  HK315-01 
LBP, Mouse, ELISA kit  1 x 96 det.   Cat.# HK205-01 
LBP, Mouse, ELISA kit  2 x 96 det.    Cat.# HK205-02 
sCD14, Human, ELISA kit  1 x 96 det.   Cat.# HK320-01 
sCD14, Human, ELISA kit 2 x 96 det.  Cat.# HK320-02 
LBP, various species, ELISA kit 1 x 96 det.  Cat.#  HK503   
EndoClear, red, small (EndoTrap red 1/1 + mini LAL)     Cat.# HIT306 
EndoClear, blue, small (EndoTrap blue 1/1 + mini LAL)     Cat.# HIT305 
IL-FABP, Human, pAb 100 µg  IA W  Cat.#HP9031   
A-FABP, Human, pAb 100 µg IA W  Cat.# HP9028  
LBP, Human, pAb 100 µg IA IP W  Cat.#HP9023 
IL-FABP, Mouse, pAb 100 µg  IA P W  Cat.#HP8011  
Lipopolysaccharide Core, mAb WN1 222-5 100 µg  FS IA P W  Cat.#HM6011
Lipopolysaccharide (LPS) from E.coli, Serotype O8:K27 1 mg  FS  Cat.# HC4065  
TLR4 agonist Set: Lipopolysaccharide (LPS) from E.coli  FS  Cat.# HC4064 

 

Posted in Scientific Publications Product News By Kim Peeters

Langerhans cells (LCs) believed to play an important role in the distribution of virus during sexual transmission of HIV.

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Posted in Scientific Publications Product News By Kim Peeters

Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase-negative granules of neutrophils. The cathelicidins are synthesized as preproproteins. Within the neutrophils...

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Posted in Scientific Publications By Kim Peeters

Items 61 to 70 of 75 total

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