IFN-alpha, Mouse, Recombinant (CHO-derived)

Catalog #: HC1040-10
Quantity: 10E6 units
$537.00

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Description
Details
This highly active interferon is constitutively produced by Chinese Hamster Ovary (CHO) cells which have been transfected with the hybrid interferon gene alpha 14-16 (alpha1 AA 1-22 and alpha 2 AA 23-166). The hybrid alpha 1, alpha 4 gene product differs 6 amino acids from alpha 4 (AA 5, 7, 10, 17, 19 and 20). The AA 10, 17, 19 and 20 influence the antiviral response. Biological activity has been demonstrated in mouse as well as in hamster and rat systems. The natural alpha 4 interferon exhibits antiviral activity in mouse cells which is approximately six times higher than that exhibited by natural alpha 1 interferon. The activity of the recombinant product is increased to a level which is approximately 100 times that of natural alpha 1 interferon and 15 times that of natural alpha 4 interferon. The situation is somewhat different in hamster cells. Here, the natural alpha 1 interferon is about 100 times more active than the natural alpha 4 interferon. The recombinant product is as active as the alpha 1 interferon.
The abovementioned features are most probably due to the fact that at least two different receptors exist for alpha interferons in these systems. One receptor appears to be specific for the amino acids 10-20 whereas the other is specific for the amino acids 55-67. The recombinant product exposes or accesses both sites better, or at least as well as the parent proteins. In addition to the interferon alpha proteins' ability to protect cells against viral infection, they can also inhibit cell growth and possess immunomodulatory activities. These effects are mediated through binding to a specific cell surface receptor. Most of the interferon alpha species are not glycosylated and are further characterized by their acid stability at pH 2.
One unit of mouse alpha interferon is the amount of alpha interferon which protects 50% of the indicator cell population from viral destruction. One unit of Hycult Biotech Mouse Alpha Interferon approximates the bioactivity contained in one unit of the mouse alpha interferon standard prepared by NIH (Ga02-901-511).
Specifications

Specifications

Catalog number HC1040-10
Product type Proteins
Quantity 10E6 units
Species Mouse
Alias Interferon-alpha, IFN-a
Formulation Lyophilized product in PBS, 100 μg mouse albumin and 0.1% gelatin containing 1.0 x 106 Units. Reconstitute the vial by pipetting 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
Storage and stability Lyophilized product should be stored at 4°C. Store stock solution after reconstitution in aliquots at -20°C. Repeated freeze and thaw cycles and prolonged exposure to light will cause loss of activity. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Infectious diseases, Tumor immunology
Application
Applications
  • Application:
    FS, IA
  • Use:
    For dilutions use protein stabilized phosphate buffered saline, pH 8 (do not use fresh serum).
References
References
References:
1. Katahira, M et al; Cytokine regulation of the rat proopiomelanocortin gene expression in AtT-20 cells. Endocrinology 1998, 139: 2414
2. Fink, K et al; Early type I interferon-mediated signals on B cells specifically enhance antiviral humoral responses. Eur J Immunol 2006, 36:2094
3. Hinson, E et al; Viperin Is Highly Induced in Neutrophils and Macrophages during Acute and Chronic Lymphocytic Choriomeningitis Virus Infection. J Immunol 2010, 184:5723
4. Rudd, P et al; Interferon Response Factors 3 and 7 Protect against Chikungunya Virus Hemorrhagic Fever and Shock. J Virol 2012, 86:9888
5. Schuessler, A et al; West Nile Virus Noncoding Subgenomic RNA Contributes to Viral Evasion of the Type I Interferon-Mediated Antiviral Response. J Virol 2012, 86:5708
6. Sriram, U et al; Myeloid Dendritic Cells from B6.NZM Sle1/Sle2/Sle3 Lupus-Prone Mice Express an IFN Signature That Precedes Disease Onset. J immunol 2012, 189:80
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