C9, Human, Natural
Catalog #: HC2142
Quantity: 250 µg
Glycoprotein complement factor C9 is synthesized as a single chain protein of 558AA and is present in human serum at ca 60ug/ml. C9 is predominantly formed in the liver, but is also synthesized by monocytes, macrophages, fibroblasts and glial cells. Pore forming proteins with a membrane-attack complex perforin domain (MACPF) are most effective to kill bacteria or virus infected cells. The MACPF domain polymerizes, refolds and inserts itself into the bilayer membrane. C5b binds to the membrane and binds C6&7. The addition of C8 to the complex triggers and accelerates C9 polymerization in order to form the asymmetric pore on the target surface. A complete pore can exist out of 12-18 C9 monomers. Together with CD59, MAC formation is regulated by eg DAF, MCP and CR1. C9 deficiency is quite common, especially in the Japanese population. These patients are more susceptible for bacterial infection.
|Formulation||0.25 ml of approximately 1 mg/ml purified natural human C9 isolated from healthy blood donors, in Phosphate buffered saline, pH 7.2. The blood donors have been tested and found negative for various viruses (see table: Human blood test results).|
|Storage and stability||Product should be stored at -70 °C. Repeated freeze and thaw cycles will cause loss of activity. The exact expiry date is indicated on the label. Use C9 protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Infectious diseases, Nephrology|