Improved ELISA for the measurement of Nitrotyrosine!
Now available, HIT501: Competitive Nitrotyrosine Assay!
Measurement of multiple NO-groups instead of one!
- Assay time: 3.5h (1h preincubation)
- Detection limit : 46.9 nM
- Dynamic range: 46.9-3000 nM
- Sample types: serum, plasma, urine, fecal extract, culture supernatant
- Sample volume: 125 μl for duplicate measurement
- Less laborious
- Less extensive pretreatment than e.g HPLC, GC-MS
- Cat. # HIT501
Measurement of nitrotyrosine
- In the average laboratory, a direct measurement of reactive oxygen species (ROS) concentrations in vivo is not possible.
- Nitrotyrosine is an indirect parameter that can determine the extent of the oxidative stress.
- Nitrotyrosine is a stable end product of peroxynitrite oxidation
- Assessment of nitrotyrosine plasma concentration may be useful as a marker of NO-dependent damage in vivo.
- Nitrotyrosine can accurately be measured using HPLC and GC-MS. However, these methods are expensive, laborious, not high throughput, and require extensive pretreatment. ELISA based methods are also possible. It is described that, so far, a competitive set-up is the most reliable.
When the formation of ROS exceeds the removal by scavenger systems, e.g. by the action of superoxide dismutase (SOD), catalase and peroxidases, this can lead to a multitude of pathological conditions:
- Parkinson’s disease
- Alzheimer’s disease
- Rheumatoid arthritis
- Inflammatory bowel diseases
- Chronic inflammation
In these diseases the protein modification can lead to modified cell-signlaing pathways, apoptosis and the creation of neoepitopes. The latter can give rise to the production of autoantibodies to e.g. complement component C1q.