Improved assay for measurement of Nitrotyrosine!

Improved ELISA for the measurement of Nitrotyrosine!

Now available, HIT501: Competitive Nitrotyrosine Assay!

Measurement of multiple NO-groups instead of one!

 Specifications:

• Assay time: 3.5h (1h preincubation)
• Detection limit : 46.9 nM
• Dynamic range: 46.9-3000 nM
• Sample types: serum, plasma, urine, fecal extract, culture supernatant
• Sample volume: 125 μl for duplicate measurement
• Non-expensive
• Less laborious
• Less extensive pretreatment than e.g HPLC, GC-MS
• Cat. # HIT501

 

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Measurement of nitrotyrosine

• In the average laboratory, a direct measurement of reactive oxygen species (ROS) concentrations in vivo is not possible.
• Nitrotyrosine is an indirect parameter that can determine the extent of the oxidative stress.
• Nitrotyrosine is a stable end product of peroxynitrite oxidation
• Assessment of nitrotyrosine plasma concentration may be useful as a marker of NO-dependent damage in vivo.
• Nitrotyrosine can accurately be measured using HPLC and GC-MS. However, these methods are expensive, laborious, not high throughput, and require extensive pretreatment. ELISA based methods are also possible. It is described that, so far, a competitive set-up is the most reliable.

Disease

When the formation of ROS exceeds the removal by scavenger systems, e.g. by the action of superoxide dismutase (SOD), catalase and peroxidases, this can lead to a multitude of pathological conditions:

• Diabetes
• Parkinson’s disease
• Alzheimer’s disease
• Rheumatoid arthritis 
• Inflammatory bowel diseases
• Chronic inflammation
• Sepsis

 In these diseases the protein modification can lead to modified cell-signlaing pathways, apoptosis and the creation of neoepitopes. The latter can give rise to the production of autoantibodies to e.g. complement component C1q.