Soluble DC-SIGN, Human, ELISA kit
DC-Sign (dendritic cell specific intracellular adhesion molecule-grabbing nonintegrin; CD209) is a receptor primarily expressed by dendritic cells (DCs).
Catalog #: HK365-01
Quantity: 1 x 96 det.
DC-Sign (dendritic cell specific intracellular adhesion molecule-grabbing nonintegrin; CD209) is a receptor primarily expressed by dendritic cells (DCs). Invading pathogens are defeated by immune cells of the innate and adaptive immune systems. The invaders are recognized by pattern recognition receptors, like TLRs and C-type lectin receptors. C-type lectins recognize carbohydrate structures on e.g. viruses and bacteria. This leads to the expression of specific cytokine which induce an adaptive Th immune response. A key C-type lectin expressed on DCs is DC-Sign. DC-Sign captures and internalizes antigen for presentation to T cells. After capturing of a pathogen via DC-Sign this leads to maturation of the DC resulting in migration from the peripheral tissue to the draining lymph node. To mediate its function in DC migration, DC-Sign is also a receptor for the recognition of self-glycoproteins, such as ICAM-3/2 as expressed by T-cells or endothelium. DC-Sign is a prototype II transmembrane receptor. DC-Sign recognizes many viruses via viral envelope glycoproteins. In some cases the virus uses this interaction to evade initiation of an adequate immune response, as known for HIV-1 infections. DC-Sign was originally discovered as HIV gp120 receptor in placenta. DC-Sign is expressed by immature DCs in peripheral tissue and mature DCs in lymphoid tissues and expression is regulated by IL-4. Besides DCs it is expressed on M2 macrophages, but not plasmacytoid DC and Langerhans cells. DC-Sign has in important role in the primary function of DCs, that is superior antigen-presenting capacity. Gene expression of DC-Sign is subjected to complex alternative splicing leading to several membrane-associated as well as soluble DC-Sign isoforms. There is little known about the expression and contribution in function of these isoforms. sDC-Sign can be found in blood, joint fluid and BAL. Expression of sDC-Sign is enhanced after CMV infection and seems to be regulated by IFN-γ and CXCL8/IL8. Although its precise function is unknown, it seems that the concentration of sDC-Sign is going down after virus infection or in cancer patients in comparison to healthy controls.
|Quantity||1 x 96 det.|
|Standard range||23-1500 pg/ml|
|Detection level||23 pg/ml|
|Working volume||100 µl/well|
|Cross reactivity||Horse - No, Mouse - No, Pig - No, Rat - No|
|Storage and stability||Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.|
Application:The Human Soluble DC-SIGN ELISA kit is to be used for the in vitro quantitative determination of Soluble DC-SIGN in plasma and serum samples.
Principle:The Human Soluble DC-SIGN ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human Soluble DC-SIGN. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Soluble DC-SIGN standards (log). The human Soluble DC-SIGN concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
The linearity of the assay was determined by serially diluting 8 plasma samples. Variation coefficient (CV) of the samples was between 1.9 and 10.2%.