H-FABP, Mouse, ELISA kit

This product is available upon request. Please contact us or your distributor for more information.
Quantity:
2 x 96 det.
Catalog #:
HK413-02

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Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas.
The H-FABP protein is derived from the FABP3 gene. The FABP content of mouse heart muscle (H-FABP) is markedly high, 10-20 mol% of cytoplasmic proteins. Following acute myocardial infarction (AMI) the small protein H-FABP is rapidly released in to the circulation. Significantly elevated serum/plasma concentrations are found within 3 h after AMI which generally return to normal values within 12 to 24 h. These features make H-FABP a useful research tool for the early assessment or exclusion of AMI, and for the monitoring of a recurrent infarction. Constitutive H-FABP released from the heart after AMI is quantitatively recovered in serum/plasma. Thus assessment of H-FABP is also a very effective tool for the estimation of the infarct size. The Mouse H-FABP kit can also be used for measurement of brain-type FABP, a marker for brain injury detection.
In serum/plasma of healthy individuals approximately 1.6 ng/ml of H-FABP is present.
Specifications
Product type Assays
Quantity 2 x 96 det.
Standard range 390-25,000 pg/ml
Detection level 400 pg/ml
Working volume 100 µl/well
Species Mouse
Alias FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor
Application The mouse H-FABP ELISA kit is to be used for the in vitro quantitative determination of mouse H-FABP in serum or plasma samples.
Principle The mouse H-FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours for Mouse H-FABP. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured mouse H-FABP. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse/rat H-FABP standards (log). The H-FABP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
References 1. Aartsen, W et al; Heart fatty acid binding protein and cardiac troponin T plasma concentrations as markers for myocardial infarction after coronary artery ligation in mice. Eur J Physiol 2000, 439: 416
2. Van der Lee, K et al; Fasting-induced changes in the expression of genes controlling substrate metabolism in the rat heart. J Lipid Res 2001, 42: 1752
3. Verges, L et al; Heart-type Fatty Acid-binding Protein Is Essential for Efficient Brown Adipose Tissue Fatty Acid Oxidation and Cold Tolerance. J of Biol Chem 2011, 286:380
Disease Cardiology and metabolism, Neurological disorders
Applications
Application assays: The mouse H-FABP ELISA kit is to be used for the in vitro quantitative determination of mouse H-FABP in serum or plasma samples.
Principle: The mouse H-FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours for Mouse H-FABP. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured mouse H-FABP. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse/rat H-FABP standards (log). The H-FABP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.
References
References: 1. Aartsen, W et al; Heart fatty acid binding protein and cardiac troponin T plasma concentrations as markers for myocardial infarction after coronary artery ligation in mice. Eur J Physiol 2000, 439: 416
2. Van der Lee, K et al; Fasting-induced changes in the expression of genes controlling substrate metabolism in the rat heart. J Lipid Res 2001, 42: 1752
3. Verges, L et al; Heart-type Fatty Acid-binding Protein Is Essential for Efficient Brown Adipose Tissue Fatty Acid Oxidation and Cold Tolerance. J of Biol Chem 2011, 286:380
Assay Manual
pdf
(Size: 0.53 MB)
Safety Data Sheet
pdf
(Size: 0.14 MB)