IL-1RI, Mouse, mAb D1f3

Quantity:
100 µg
Catalog #:
HM1024-100UG

Original supplier of innate immunity products since 1994.

Our commitment to quality: Read more!

Contact our support team for more product information.

Can't find your application for a product? Request a sample!

The monoclonal antibody binds to soluble mouse interleukin-1 receptor type I. The IL-1 system includes two agonists (IL-1alpha and IL-1beta), converting enzymes, antagonists, two receptors (IL-1RI and IL-1RII) and the IL-1 receptor accessory protein. Interleukin-1 signal is transduced through the type I receptor.
Specifications
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Application Functional studies, Western blot
Use For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts IL-1 RI to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
Isotype Rat IgG1
Species Mouse
References 1. Kojouharoff, G et al; Neutralization of tumour necrosis factor (TNF) but not of IL-1 reduces inflammation in chronic dextran sulphate sodium induced colitis in mice. Clin Exp Immunol 1997, 107: 353
2. Stern, A et al; Purification to homogeneity and amino acid sequence analysis of a receptor protein for interleu- kin-1. Arch Biochem Biophys 1989, 274: 26
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity, Neurological disorders
Applications
Application: Functional studies, Western blot
Application notes: The antibody can be used for Western blotting. Furthermore the antibody is useful for inhibition in biological assays, in vitro as well as in vivo. Although for in vitro use the monoclonal antibody HM1022 (clone Reg21) is recommended.
Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts IL-1 RI to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
References
References: 1. Kojouharoff, G et al; Neutralization of tumour necrosis factor (TNF) but not of IL-1 reduces inflammation in chronic dextran sulphate sodium induced colitis in mice. Clin Exp Immunol 1997, 107: 353
2. Stern, A et al; Purification to homogeneity and amino acid sequence analysis of a receptor protein for interleu- kin-1. Arch Biochem Biophys 1989, 274: 26
CoA-TDS HM1000-HM1066
pdf
(Size: 0.22 MB)
Protocol
pdf
(Size: 0.09 MB)