CD180, Mouse, mAb RP/14

Quantity:
100 µg
Catalog #:
HM1031-100UG

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Radioprotective 105 (RP105, CD180) is a type 1 transmembrane protein of 105 kDa with extracellular leucine-rich repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. Radioprotective 105 (RP105, CD180) expression is largely restricted to immune cells including CD19+ B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (RP/14) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule B7.2, revealing RP105 as a potent regulator of B-cell activation. RP105 (CD180) has an important role in B-cell activation by LPS. It is important to note that RP105 associates with MD1, which is in this context important for RP105 with respect to B-cell surface expression and LPS recognition and signalling. MD-1 seems to be requisite for efficient expression of RP105.
Specifications
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Application Flow cytometry, Functional studies
Use For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Isotype Rat IgG2a
Species Mouse
Alias RP105, Ly78
References 1. Miyake, K et al; Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 1998, 161: 1348
2. Nagai, Y et al; Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood 2002, 99: 1699
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease Autoimmunity, Tumor immunology
Applications
Application: Flow cytometry, Functional studies
Application notes: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Use: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References: 1. Miyake, K et al; Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 1998, 161: 1348
2. Nagai, Y et al; Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood 2002, 99: 1699
CoA-TDS HM1000-HM1066
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Protocol