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Podoplanin, Mouse, clone pMab-1

Podoplanin (PDPN), also known under the name Aggrus, is highly expressed in various tumors (such as oral, lung, esophageal, brain) and in normal cells as lymphatic endothelial cells and podocytes.
Catalog #: HM1141
Quantity: 100 µg
$384.00

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Description
Details
Monoclonal antibody pMab-1 recognizes human Podoplanin (PDPN; agrrus). Podoplanin is a small type_I transmembrane sialoglycoprotein expressed on a broad range of cell types and involved in platelet aggregation and tumor metastasis. The ca 38 kDa mouse protein was initially identified as a biomarker of lympatic endothelium, alveolar epithelium and glomerular podocytes. Expression has also been found in many tumors. However the functional role of PDPN is still poorly understood. Podoplanin consists of an extracellular domain, transmembrane domain and a cytoplasmic tail. In its extracellular domain it possesses a platelet aggregation stimulating (PLAG) domain. Podoplanin has got three PLAG domains of which PLAG3 is critical for binding with C-type lectin receptor-2 (CLEC-2). This PDPN specific receptor mediates platelet aggregation. This interaction is required to initiate and maintain separation of blood and lymphatic vessels and can be critical in process of cancer metastasis. This may lead to PDPN as possible therapeutic target. Antibody PMab-1 is produced against the platelet aggregation-stimulating domain of mouse PDPN and can be applied in western blotting, FACS and immunohistochemistry. Administration of PMab-1 in vivo reduces lymphangiogenesis in the corneal suture and ear wound healing models. PMab-1 also suppresses the infiltration of thioglycollate-induced macrophages at the site of wound healing. Furthermore, administration of PMab-1 leads to a significant suppression of the rejection reaction in the corneal transplantation model.
Specifications

Specifications

Catalog number HM1141
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 µg/ml) 0.2 µm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin.
Immunogen Synthetic peptides, corresponding to amino acids 38–51 of mouse podoplanin (Ref. 1)
Isotype Rat IgG2a
Species Mouse
Alias Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Application
Applications
  • Application:
    F, FC, FS, IF, P, W
  • Application Notes:
    IHC-P: High temperature citrate pretreatment was used. Fixing the tissue longer than 24 hours leads to masking or destruction of podoplanin antigen.
    W: A reduced or non-reduced sample treatment and SDS-Page can be used. The band size is ~40 kDa.
References
References
References:
1. Kaji, C et al; Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin. Acta Histo Cyto 2012, 45: 227
2. Maruyama, Y et al; The Effect of Podoplanin Inhibition on Lymphangiogenesis Under Pathological Conditions. Inves Opt & Vis Science 2014, 55: 4813
3. Fujii Y, et al. MAP tag: A novel tagging system for protein purification and detection. Monoclon. Antib. Immunodiagn. Immunother 2016, 35:: 293
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