IFN-gamma, Human, mAb F12

Quantity:
300 µg
Catalog #:
HM2003-300UG

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Monoclonal antibody binds and neutralizes both natural and recombinant human gamma Interferon. Cross reactivity with other cytokines has not been found. The antibody does not react with rodent interferons. Possible applications include the purification of human gamma Interferon and in vitro neutralisation. In general, monoclonal antibodies to gamma Interferon are able to inhibit Schwartzman reactions and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease.
Specifications
Product type Monoclonal antibodies
Quantity 300 µg
Formulation Lyophilized product in PBS, containing 300 µg antibody. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).
Application Frozen sections, Functional studies, Immuno assays, Immuno precipitation
Use For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated. One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23-901-530).
Isotype Mouse IgG1
Species Human
References 1. Armstrong, JA et al; Semi-micro, dye-binding assay for rabbit interferon. J Appl Microbiol 1971, 21: 723
2. Billiau, A et al; Gamma-interferon : the match that lights the fire ? Immunol Today 1988, 9: 37
3. Jacob, CO et al; In vivo treatment of (NZB X NZW) F1 lupus-like nephritis with monoclonal antibody to gamma interferon. J Exp Med 1987, 166: 798
4. Meide van der, PH et al; Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA. J Immunol Methods 1985, 79: 293
Storage and stability Lyophilized product should be stored at 4 °C. Store stock solution in aliquots at -20 °C. Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease Autoimmunity, Infectious diseases
Applications
Application: Frozen sections, Functional studies, Immuno assays, Immuno precipitation
Use: For immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated. One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23-901-530).
References
References: 1. Armstrong, JA et al; Semi-micro, dye-binding assay for rabbit interferon. J Appl Microbiol 1971, 21: 723
2. Billiau, A et al; Gamma-interferon : the match that lights the fire ? Immunol Today 1988, 9: 37
3. Jacob, CO et al; In vivo treatment of (NZB X NZW) F1 lupus-like nephritis with monoclonal antibody to gamma interferon. J Exp Med 1987, 166: 798
4. Meide van der, PH et al; Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA. J Immunol Methods 1985, 79: 293
CoA-TDS HM1067-HM2099
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