H-FABP, Human, mAb 67D3, biotinylated

Quantity:
50 µg
Catalog #:
HM2018BT-50UG

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The monoclonal antibody 67D3 recognizes human heart-type fatty acid-binding protein (H-FABP) of both natural and recombinant origin. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. Furthermore, this antibody is useful for the purification of H-FABP.
Specifications
Product type Monoclonal antibodies
Quantity 50 µg
Formulation 0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Conjugate Biotin
Application Immuno assays, Immuno precipitation, Western blot
Use For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen Purified Human H-FABP
Isotype Mouse IgG1
Species Human
Cross reactivity Mouse - Yes, Rat - Yes
Alias Fatty acid-binding protein 3, FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor
References 1. Roos, W et al; Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth 1995, 183: 149
2. Pelsers, M et al; Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility. Clin Chem 2004, 50: 1568
3. Zhen, E et al; Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necrosis. Proteomics Clin Appl 2007, 1: 661
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hbt is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease Cardiology and metabolism, Neurological disorders
Applications
Application: Immuno assays, Immuno precipitation, Western blot
Application notes: IP: Biotinylated 67D3 was immobilized on streptavidin beads and added to serum to immunoprecipitate H-FABP (Ref.3).
W: Reduced sample treatment. The band size is ~15 kDa (Ref.2).
Use: For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Positive control: Heart cells, recombinant human H-FABP
References
References: 1. Roos, W et al; Monoclonal antibodies to human heart fatty acid-binding protein. J Immunol Meth 1995, 183: 149
2. Pelsers, M et al; Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility. Clin Chem 2004, 50: 1568
3. Zhen, E et al; Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necrosis. Proteomics Clin Appl 2007, 1: 661
CoA-TDS HM1067-HM2099
pdf
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Protocol
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