DC-SIGN, Human, mAb DCN47.5

Catalog #: HM2209
Quantity: 100 µg
$474.00

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Description
Details
The monoclonal antibody DCN47.5 reacts with the C-type lectin, DC-SIGN (CD209), exclusively expressed on human dendritic cells (DC). DC are specialized antigen presenting cells and bridge the innate and the adaptive immune system. They provide high levels of costimulation necessary for activation of both naïve and antigen-experienced T-cells. Immature DC are capable to migrate to inflammatory sites, capture antigen and process it internally to form MHC-peptide complexes. Following antigen uptake, DC undergo maturation and migrate to lymphoid organs where they can present MHC-peptide complexes to resting T-cells and drive T-cell proliferation. During differentiation and maturation of DC, several phenotypic surface markers are expressed: CD1a, CD4, CD11, CD40, CD86, and HLA-DR. Immature DC predominantly express CCR5 which enables DC to migrate to inflammatory sites, whereas mature DC express high levels of CXCR4, a receptor that facilitates migration to lymphoid organs.
DC also express DC-specific, ICAM-3 grabbing, nonintegrin (DC-SIGN), a 44 kDa C-type lectin that binds to the HIV-1 envelope glycoprotein gp120, ICAM-3 on T-cells and ICAM-2 on endothelial cells. HIV virions are able to infect cells expressing CD4 and the chemokine co-receptors CCR5 or CXCR4 and can attach to DC-SIGN to extend virion lifespan. Therefore, DC are candidates for HIV-1 infection. DC-SIGN-ICAM-3 binding is integrin-independent but calcium-dependent and antibodies reactive against DC-SIGN can be used to study DC-SIGN-ICAM3 binding.
The monoclonal antibody DCN47.5 specifically reacts with the C-type lectin DC-SIGN (CD209) expressed on human dendritic cells and inhibits binding of DC-SIGN to ICAM-2 on endothelial cells.
Specifications

Specifications

Catalog number HM2209
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS containing 0.1% bovine serum albumin.
Immunogen Human monocyte-derived dendritic cells
Isotype Mouse IgG1
Species Human
Alias CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1
Gene name: CD209
Storage and stability Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease Infectious diseases
Application
Applications
  • Application:
    FC, FS
  • Positive Control:
    human lymphnodes or PHA activated T cells
  • Use:
    Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
References
References
References:
1. Leeuwenberg, JFM et al; Slow release of soluble TNF-Receptors by monocytes in vitro. J Immunol 1994, 152: 4036
2. Leeuwenberg, JFM et al; Lipopolysaccharide LPS-mediated soluble TNF-Receptor release and TNF-Receptor expression by monocytes; role of CD14, LPS binding protein and bactericidal/permeability-increasing protein. J Immnol 1994, 152: 5070
3. Marchetti, L et al; Tumor necrosis factor (TNF)-mediated neuroprotection against glutamate-induced excitotoxicity is enhanced by N-methyl-D-aspartate receptor activation. Essential role of a TNF receptor 2-mediated phosphatidylinositol 3-kinasedependent NF-kappa B pathway. J Biol Chem 2004, 279: 32869
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