Coagulation factor VII, Human, mAb CLB VII-1

Catalog #: HM2347
Quantity: 100 µg
$332.00

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Description
Details
Antibody clone CLB VII-1 recognizes human coagulation factor VII. FVII is a serine protease-coagulating element responsible for the initiation of an extrinsic pathway of coagulation. FVII is vitamin K dependent and is synthesized by hepatocytes. It is a single chain glycoprotein of ca 50kDa, consisting of 408 amino acids. The protein has several functional domains, a gamma-carboxyglutamic acid-rich domain at the N-terminus, 2 EGF-like domains and the C-terminal serine protease domain. FVII itself has no real activity. Activation of FVII leads to FVIIa. At the site of injury, blood clotting starts by interaction of FVII with tissue factor (TF). Complexed with Ca2+, FVIIa/TF activates FIX and FX, which ultimately leads to the formation of a fibrin clot.
FVII deficiency is a rare haemological disorder with an incidence of 1/500000. Disease is characterized by multiple clinical phenotypes, from asymptomatic to life threatening bleedings. FVII levels can be measured by ELISA of turbidimetry, although plasma levels are low (0.5μg/ml). Plasma levels can be influenced by gene polymorphisms.
Specifications

Specifications

Catalog number HM2347
Product type Monoclonal antibodies
Quantity 100 µg
Formulation 1 ml (100 μg/ml) 0.2 μm filtered protein A purified antibody solution in 20 mM TRIS, 150 mM NaCL, pH 8.0.
Immunogen Mixture of Human Factor VII and Factor VIIa
Isotype Mouse IgG1
Species Human
Alias Blood-coagulation factor VIIa, activated blood coagulation factor VII, formerly known as proconvertin
Storage and stability Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Precautions For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease Autoimmunity, Cardiology and metabolism
Application
Applications
  • Application:
    W
  • Application Notes:
    W: A reduced and non-reduced sample treatment and SDS-Page was used. The band size is 70 kDa.
  • Use:
    Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions.
References
References
References:
1. Berckmans RJ et al. Cell-derived Microparticles Circulate in Healthy Humans and Support Low Grade Thrombin Generation. Thromb Haemost 2001, 85: 639
2. Berckmans RJ et al. Cell-derived micro particles in synovial fluid from inflamed arthritic joints support coagulation exclusively via a factor Vll-dependent mechanism. Arthritis Rheum 2002, 46:2857
3. Berckmans RJ et al; Cell-derived vesicles exposing coagulant tissue factor in saliva. Blood 2011, 117:3172
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