LAIR-1, Human, mAb NKTA255, functional study antibody, low endotoxin
Catalog #: HM2364-FS
Quantity: 0.5 mg
The monoclonal antibody clone NKTA255 recognizes human leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1). The LAIR family consist of at least two members; LAIR-1 (CD305) and LAIR-2 (CD306). To date, several splice variants of LAIR-1 have been identified. LAIR-1 is an inhibitory receptor expressed on most immune cells, like NK cells, T- & B-cells, monocytes, DCs, megakaryocytes, eosinophils, basophils, and mast cells. Especially the expression on pDCs is high. The main ligand of LAIR proteins is collagen. LAIR-1 is a transmembrane receptor with a single extracellular Ig-like domain, a transmembrane region and a cytoplasmic tail. This tail consist of two immunoreceptor tyrosine-based inhibition motif (ITIM) domains, which are responsible for its immunomodulating action. After cross-linking, the tyrosines in the ITIMs become phosphorylated which is required for inhibition of cellular activation. By shedding a soluble variant of the protein arises. sLAIR-1 can be found in healthy individuals and can be used as a marker of lymphocyte activation. sLAIR-1 has been found among others in serum, urine, amniotic fluid, and synovial fluid. Besides collagen, a few other ligands are described like Complement C1q, MBL, and SP-D. Complement activation of the classical pathway is controlled by C1q. By binding of LAIR-1 to C1q, pathway initiation is hampered leading to diminished complement related phagocytosis and immune regulation. The protein has been associated with several (auto-) immune diseases. In rheumatoid arthritis expression is decreased on CD4+ T-cells and high in CD14+ monocytes synovial macrophages. Also in SLE lower levels of LAIR-1 has been found on pDCs. sLAIR-1 might be an indicator of the immune response after virus infection or organ transplants. LAIR-1 modulates apoptosis and cytokine secretion in THP-1 cells.
|Product type||Monoclonal antibodies|
|Formulation||0.5 mg of 0.2 μm filtered protein A purified antibody solution in PBS with a concentration of at least 0.5 mg/ml. The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay.|
|Immunogen||Activated NK cells and CD3- thymocytes.|
|Alias||Leukocyte-associated immunoglobulin-like receptor 1, hLAIR1, CD305 Gene name: LAIR1, CD305|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Disease||Autoimmunity, Infectious diseases|
Application:FC, FS, IA, IP, W
Application Notes:IA: HM2364 can be used as both capture and detection antibody. W: A non-reduced and reduced sample treatment and SDS-Page was used. The band size is ~31 kDa.
Use:Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
1. Zocchi, M et al; Leukocyte-associated Ig-like receptor-1 prevents granulocyte-monocyte colony stimulating factor-dependent proliferation and Akt1/PKB alpha activation in primary acute myeloid leukemia cells. Eur J Immunol 2001, 31:3667 2. Colombo, B et al; Defective Expression and Function of the Leukocyte Associated Ig-like Receptor 1 in B Lymphocytes from Systemic Lupus Erythematosus Patients. PLosONE 2012, 7: e31903