HBD2, Human, clone HB5C10
Monoclonal antibody HB5C10 recognizes human beta defensin-2 (HBD2). hBD-2 is a cystein-rich cationic 41 amino acid antimicrobial peptide of 4-5 kDa.
Monoclonal antibody HB5C10 recognizes human beta defensin-2 (HBD2). hBD-2 is a cystein-rich cationic 41 amino acid antimicrobial peptide of 4-5 kDa. Human BD-2 is produced by epithelial cells upon stimulus by lipopolysaccharides and proinflammatory cytokines TNFα and IL1β. Contact of keratinocytes with gram-negative bacteria results in rapid induction of hBD-2 protein. hBD-2 has been described as a dynamic component of the local epithelial defense system of the skin, intestinal and respiratory tract, where it functions by protecting surfaces from infection. Its local expression has been associated with skin lesions like psoriasis as well as infected lung epithelia of patients with cystic fibrosis. Furthermore, in inflammatory bowel disease (IBD), expression of hBD-2 is increased in patients with IBD compared to healthy persons. The NF-kB pathway has been recognized as a key component in the induction of hBD-2 expression, however, other studies have observed induction mediated by the mitogen-activated protein kinase (MAPK) pathways. Thus, increased expression of hBD-2 in epithelial cells is associated with the proinflammatory response. This is supported by the finding that the anti-inflammatory cytokines IL-10 and IL-13 downregulate the synthesis of hBD-2 in atopic dermatitis.
|Product type||Monoclonal antibodies|
|Formulation||0.2 µm filtered in PBS+0.1%BSA+0.02%NaN3|
|Application||Immuno assays, Western blot|
|Use||Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.|
|Alias||Human Defensin B 2|
|Storage and stability||Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.|
|Precautions||For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.|
|Application:||Immuno assays, Western blot|
|Application notes:||- IA: Antibody HB5C10 can be used as capture and detection antibody.
- W: western blot was performed under non-reducing conditions.
|Use:||Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.|