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Catalog # HM5005

Ethenoadenosine, mAb 1G4

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€125.00 – €481.00

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The reaction of chloroacetaldehyde (CAA) with DNA results in the formation of ethenoadenosine also known as 1,N6-ethenodeoxy-adenosine or etheno-A. CAA is formed through metabolization of for example vinyl chloride, a well established carcinogen. In RNA, after in vitro activation, etheno-A and etheno-C are the principle products of RNA damage. Etheno derivate formation may be highest in single-stranded DNA-regions. The monoclonal antibody 1G4 reacts with both the ribose and deoxyribose form of the adduct. The antibody is not cross reactive with non-modified DNA or the normal nucleotides.

Application
Flow cytometry, Frozen sections, Immuno assays, Immuno precipitation, Western blot

Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Isotype
Mouse IgG2
Species
N/A
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Tumor immunology
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol Immuno precipitation
54 kb
Protocol Flow Cytometry
58 kb
Protocol IHC-F
36 kb
CoA-TDS HM4000-6000
HM5005
47 kb
HM5005-20UG
47 kb
Safety Data Sheet
SDS - HM5005-100UG
52 kb
SDS - HM5005-20UG
52 kb

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