LPS ligands

LPS Ligands – TLRclassified Ligands!

The LPS ligands in our product range are TLRclassified:

– High purity (>98%)

– LPS lacks any detectable bacterial, (lipo-)protein, DNA, RNA or other TLR-stimulating activity due to its ultra-purified formulation

– In sterile solution

Experiment with HC4045: Lipopolysaccharide (LPS) from E.coli Serotype O55:B5

Macrophagesfrom TLR deficient (TLR4 knock-out) mice and from TLR4 expressing wild-type (WT) were stimulated with E.coli Serotype O55:B5, in a concentration of 1 µg/ml.

After 24 hours, the cell culture supernatant was analyzed by measuring IL-6 with ELISA.

Recommended range for S-type (wild-type) LPS:  0.01 – 1.0 µg/ml.

Optimal concentrations required for activation depend upon bacterial strain and chemotype (R- or S-) LPS, cell species, cell culture conditions, sampling time and cytokine.

Two antibodies, LPScore (cat.# HM6011) and to Lipid A (cat.# HM6009) were used in an experiment with several LPS types. Both antibodies have the capability to recognise several LPS types:

 

   

 

	LPS Ligand cat.#	HM6009		HM6011
LPS 1. Serratia Marcescero		–		–
LPS 2. P. Aeruginosa type 10		–		+
LPS 3. E.coli O55:B5	HC4045	+		+
LPS 4. E.coli O41:B4		–		++
LPS 5. E.coli O128:B12		+		+
LPS 6. E.coli O127:B8		–		++
LPS 7. S. Typhosa		+		++
LPS 8. E.coli O26:B6		++		++
LPS 9. Salmonella Abortus Equi	HC4061	++		–
LPS 10. S.enteriditis	HC4059	–		++
LPS 11. E. coli		++		++
LPS 12. K.pneumoniae		++		–
LPS 13. P.aeruginosa		++		++
LPS 14. S.typhimurium	HC4060	+		++