LPS Ligands – TLRclassified Ligands!

The LPS ligands in our product range are TLRclassified:

High purity (>98%)

– LPS lacks any detectable bacterial, (lipo-)protein, DNA, RNA or other TLR-stimulating activity due to its ultra-purified formulation

– In sterile solution

Experiment with HC4045: Lipopolysaccharide (LPS) from E.coli Serotype O55:B5

Macrophagesfrom TLR deficient (TLR4 knock-out) mice and from TLR4 expressing wild-type (WT) were stimulated with E.coli Serotype O55:B5, in a concentration of 1 µg/ml.

After 24 hours, the cell culture supernatant was analyzed by measuring IL-6 with ELISA.

Recommended range for S-type (wild-type) LPS:  0.01 – 1.0 µg/ml.

Optimal concentrations required for activation depend upon bacterial strain and chemotype (R- or S-) LPS, cell species, cell culture conditions, sampling time and cytokine.

HC4045

Two antibodies, LPScore (cat.# HM6011) and to Lipid A (cat.# HM6009) were used in an experiment with several LPS types. Both antibodies have the capability to recognise several LPS types:

 

   

 

 

LPS Ligand cat.#

HM6009

 

HM6011

LPS 1. Serratia Marcescero

 

 

LPS 2. P. Aeruginosa type 10

 

 

+

LPS 3. E.coli O55:B5

HC4045

+

 

+

LPS 4. E.coli O41:B4

 

 

++

LPS 5. E.coli O128:B12

 

+

 

+

LPS 6. E.coli O127:B8

 

 

++

LPS 7. S. Typhosa

 

+

 

++

LPS 8. E.coli O26:B6

 

++

 

++

LPS 9. Salmonella Abortus Equi

HC4061

++

 

LPS 10. S.enteriditis

HC4059

 

++

LPS 11. E. coli

 

++

 

++

LPS 12. K.pneumoniae

 

++

 

LPS 13. P.aeruginosa

 

++

 

++

LPS 14. S.typhimurium

HC4060

+

 

++

         
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