Mouse SAP ELISA

Now Available! Mouse SAP ELISA

For the Mouse SAP Manual click here…

Specifications:

  • Working time of 3½ hours.
  • Minimum concentration which can be measured is 0.4 ng/ml.
  • Measurable concentration range of 0.4 to 100 ng/ml.
  • Working volume of 100 µl/well.
  • Suitable for serum and plasma samples
  • Available in 1- and 2-plates (Cat.# HK215-01 and HK215-02)

Mouse SAP:

  • Mouse SAP has shown to be a reliable marker of the acute phase.
  • In mouse, SAP levels increase significantly 24 hours after challenge with lipopolysaccharide.
  • The normal mouse serum level of SAP is similar between males and females, but is different among mouse strains. Endogenous SAP levels range from 20 µg/ml to 150 µg/ml.

General SAP information:

  • Serum amyloid P component (SAP) belongs to a highly conserved superfamily of calcium-dependent ligand binding and lectin (carbohydrate binding) proteins, also called pentraxins, due to the pentameric structure of the proteins.
  • The polypeptide subunits are 31 kDa in size under reducing conditions.
  • The C-reactive protein (CRP) and SAP are  short pentraxins, which are produced in the liver in response to inflammatory mediators. Pentraxin 3 (PTX3), is the prototypic long pentraxin.
  • Both SAP and CRP are evolutionary conserved in all vertebrates and also found in distant invertebrates such as the horseshoe crab (Limulus polyphemus).
  • SAP contributes significantly to the pathogenesis of amyloidosis. The mechanism of its participation is not yet known and may differ between species.
  • Human SAP and CRP share 51% residue sequence identity and 66% homology.

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