Home \ Assays \ Arginase-I, Human, ELISA kit
Catalog # HK386

Arginase-I, Human, ELISA kit

810,001.308,00

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Arginase-I (liver-type Arginase, L-arginine ureahydrolase, or L-arginine amidinohydrolase) is an hydrolytic enzyme that converts arginine to ornithine and urea. Ornithine is the precursor for preline, an essential amino acid for collagen synthesis and for poly-amines, key components in cell growth and differentiation.
Arginase exists as two forms designated Arginase-I and Arginase-II which are encoded by different genes. Although Arginase-I and Arginase-II have similar enzyme activities, they differ in immunological reactivity. Human Arginase-I (liver-type Arginase) is a 35 kDa protein circulating in blood probably as a homotrimer. Liver-type Arginase is most abundant expressed in mammalian liver, but is also found in non-hepatic tissues, for instance red blood cells, lactating mammalian glands, and the kidney. In addition to its involvement in ammonia detoxification via the urea cycle, Arginase plays a role in other processes, for instance macrophage-mediated cytotoxicity due to Arginase release and inhibition of lymphocyte proliferation in vitro. It shows high activity in growing tissues, wound healing, proliferating lymphocytes and tumors. Furthermore, Arginase acts as a modulator of the immune response. Besides this Arginase plays a role in allergen challenged lungs, in autoimmune inflammation in the central nervous system and in acute liver injury.

Application
The human Arginase-I ELISA kit is to be used for the in vitro quantitative determination of human Arginase-I in serum and plasma samples.

Principle
The human arginase-I ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured human arginase-I. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the arginase-I standards (log). The human arginase-I concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Linearity
The linearity of the assay was determined by serially diluting 4 samples. Variation coefficient (CV) of the samples was between 4.2 and 9.7%.

Product type
Assays
Quantity
1 x 96 det., 2 x 96 det.
Standard range
1.6-100 ng/ml
Detection level
1.6 ng/ml
Working volume
100 µl/well
Species
Human
Cross reactivity
Mouse – No, Pig – No, Rat – No
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Autoimmunity, Tumor immunology
Safety Data Sheet
HK386
60 kb
Assay Manual
HK386
349 kb

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