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Catalog # HP9060

Caspase-3, Human, pAb

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The caspase-3 antibody recognizes caspase-3, a member of the peptidase C14A family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Caspase-3 is considered to be the major executioner caspase; that is, the primary downstream mediator of apoptotic associated proteolysis. Human pro-caspase-3 is a 32 kDa, 277 amino acid (aa) protein. Normally, it is an inactive, cytosolic homodimer, but following an upstream signal that activates processing proteases, pro-caspase-3 undergoes proteolytic cleavage. Multiple proteases can use caspase 3 as a substrate including caspase-6, -8, and -10, granzyme B, and caspase-3 itself. Active caspase-3 is known to utilize a Cys residue to cleave multiple substrates, including PARP, proIL16, PKCγ & δ, pro-caspases-6, -7 and -9, and βcatenin. Caspase-3 the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer’s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. This caspase-3 polyclonal antibody detects endogenous levels of caspase-3 protein.

Immuno assays, Immuno fluorescence, Western blot

Application Notes
W: A reduced and non-reduced sample treatment and SDS-Page was used. The band size is ~17 kDa.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Polyclonal antibodies
100 µg, 20 µg
0.1 ml (1 mg/ml) 0.2 μm filtered antibody solution in PBS, containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Synthesized peptide derived from human Caspase-3 around the non-phosphorylation site of S150
Rabbit Ig
Cross reactivity
Mouse – Yes, Rat – Yes
CASP-3, Apopain, Cysteine protease CPP32, CCP-32, Protein Yama, SREBP cleavage activity 1, SCA-1 </br> - Gene name: CASP3, CCP32
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Infectious diseases
78 kb

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