Home \ Antibodies \ Caspase-8, Human, pAb
Catalog # HP9061

Caspase-8, Human, pAb

333,00

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The caspase-8 antibody recognizes caspase-8, member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. The caspase-8 polyclonal antibody recognizes endogenous caspase-8.

Application
Immuno assays, Western blot

Application Notes
W: A reduced and non-reduced sample treatment and SDS-Page was used. The band size is ~55 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Polyclonal antibodies
Quantity
100 µg
Formulation
0.1 ml (1 mg/ml) 0.2 μm filtered antibody solution in PBS, containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunogen
Synthesized peptide derived from human Caspase-8 around the non-phosphorylation site of S347
Isotype
Rabbit Ig
Species
Human
Cross reactivity
Rat – Yes
Alias
CASP-8, Apoptotic protease Mch-5, Apoptotic cysteine protease, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, ICE-like apoptotic protease 5, MACH </br> - Gene name: CASP8, MCH5
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Infectious diseases
CoA-TDS pAb
HP9061
80 kb

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