Home \ Antibodies \ CD11/CD18 (LFA-1), Human, mAb 24
Catalog # HM2183

CD11/CD18 (LFA-1), Human, mAb 24


Please choose the quantity of items you wish to add to your cart…
Find distributor

The monoclonal antibody 24 recognizes an epitope of 174 kD present on leukocyte integrins, CD11/CD18 (LFA-1)
or beta 2-type integrins. The leukocyte integrins are a family of heterodimeric receptors that mediate
divalent cation-dependent cellular adhesion reactions. T cells use integrins in essentially all of their
functions. Integrins become active following signalling through other membrane receptors, which cause
both affinity alteration and an increase in integrin clustering. The monoclonal antibody 24 recognizes a
structural feature and is strictly dependent upon the presence of Mg2+. The epitope is located within, or in
close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to
the leukocyte integrins and thus reflects functionally active molecules. The epitope can be induced on
polymorphonuclear leukocytes and on monocytes. Glu173 and Glu175 of the beta(2) I domain are identified
as critical for antibody 24 binding.
The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2-
activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell
proliferation. Furthermore the monoclonal antibody 24 prevents “deadhesion” of receptor/ligand pairs,
possible locking leukocyte integrins in an “active” conformation.

Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation

For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts activated CD11/CD18 to be inhibited.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Mouse IgG1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Infectious diseases, Pulmonology

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Contact us