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Catalog # HM3029

CD68, Rat, mAb ED1

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The monoclonal antibody ED1 recognises a single chain heavily glycosylated protein of 90-110 kD (depending on the cell type as antigen source) that is expressed on the lysosomal membrane of phagocytes as well as on the cell surface (weak expression). This antigen is the rat homologue of human CD68. The expression of this antigen in cells increases during phagocytic activity. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. This makes the monoclonal antibody ED1 a useful marker for rat macrophages. The monoclonal antibody ED1 is not able to block latex phagocytosis or bacterial killing.

Application
Flow cytometry, Frozen sections, Immuno precipitation, Paraffin sections, Western blot

Use
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
20 µg, 100 µg
Formulation
0.2 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Isotype
Mouse IgG1
Species
Rat
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Tumor immunology

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