CD83, Human, mAb ELBE-1

Monoclonal antibody ELBE-1 recognizes human CD83. CD83 is a cell surface marker predominantly
expressed on mature DCs. Expression has been found on circulating and tissue DCs. Additionally CD83
is found on activated B and T lymphocytes. Human CD83 is composed of 205 amino acids but has, due
to extensive glycosylation, a molecular weight of ca 45 kDa. CD83 is expressed as a conserved a
noncovalently associated single chain molecule membrane protein. CD83 is considered a marker for
fully matured dendritic cells in the peripheral circulation. An essential role has not been found. However,
its function primarily lies in the regulation of T- and B-cell maturation and the regulation of their
peripheral responses. CD83 is supposed to function as a co-stimulator for T-cell activation similar to
CD80 or CD86. Enhanced expression on DCs correlates with their T cell activating capacity. Besides
the membrane version of the protein also a soluble form occurs (sCD83). sCD83 levels are raised in
sera from patients of chronic lymphocytic leukemia and other haematological malignant diseases and
in the synovial fluid of rheumatoid arthritis. While the membrane bound form has immune stimulatory
capacity, the soluble form has the opposite effect and is considered to have immunosuppressive
potential. Due to its inhibitory functions, sCD83 has potential for treatment of eg. autoimmune diseases
and transplantation.
In contradiction to most clones, antibody ELBE-1 is besides flow cytometry applicable in western blotting
and ELISA (detecting of sCD83). ELBE-1 recognizes a linear epitope and doesn’t influence T cell
activation.