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Catalog # HM2321

CD83, Human, mAb ELBE-1


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Monoclonal antibody ELBE-1 recognizes human CD83. CD83 is a cell surface marker predominantly
expressed on mature DCs. Expression has been found on circulating and tissue DCs. Additionally CD83
is found on activated B and T lymphocytes. Human CD83 is composed of 205 amino acids but has, due
to extensive glycosylation, a molecular weight of ca 45 kDa. CD83 is expressed as a conserved a
noncovalently associated single chain molecule membrane protein. CD83 is considered a marker for
fully matured dendritic cells in the peripheral circulation. An essential role has not been found. However,
its function primarily lies in the regulation of T- and B-cell maturation and the regulation of their
peripheral responses. CD83 is supposed to function as a co-stimulator for T-cell activation similar to
CD80 or CD86. Enhanced expression on DCs correlates with their T cell activating capacity. Besides
the membrane version of the protein also a soluble form occurs (sCD83). sCD83 levels are raised in
sera from patients of chronic lymphocytic leukemia and other haematological malignant diseases and
in the synovial fluid of rheumatoid arthritis. While the membrane bound form has immune stimulatory
capacity, the soluble form has the opposite effect and is considered to have immunosuppressive
potential. Due to its inhibitory functions, sCD83 has potential for treatment of eg. autoimmune diseases
and transplantation.
In contradiction to most clones, antibody ELBE-1 is besides flow cytometry applicable in western blotting
and ELISA (detecting of sCD83). ELBE-1 recognizes a linear epitope and doesn’t influence T cell

Flow cytometry, Immuno assays, Western blot

Application Notes
FC: Antibody ELBE-1 stains the extracellular domain of CD83. (Ref.1)
W: A non-reduced and/or reduced sample treatment and SDS-Page was used. The band size is 40kDa kDa (Ref.1).

For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Positive Control
PMBCs Double staining with CD11c-PE

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and - 0.02% sodium azide.
huCD83Ig fusion protein
Mouse IgG1
B-cell activation protein, Cell surface protein HB15
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least - one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility - of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is - not responsible for any patent infringements that might result from the use or derivation of this product.
Autoimmunity, Tumor immunology

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