DNAX accessory molecule-1 (DNAM-1) is a 65 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. DNAM-1 is constitutively expressed on a majority of T cells, NK cells and macrophages. Interaction of DNAM-1 with DNAM-1 ligands CD155 and CD112 induces intercellular adhesion, infiltration and cell signaling, resulting in a variety of cellular responses including innate and adaptive immunities.
Flow cytometry, Functional studies
FC: Antibody TX92 stains the extracellular domain of DNAM-1. FS: Antibody TX92 has an inhibitory function of DNAM-1 binding to the ligands. It additionally causes partial depletion of PB CD8+ T cells (Ref.1).
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:25. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.