The monoclonal antibody ECE.2 recognizes mouse monocyte chemoattractant protein 1 (MCP-1).
The murine JE gene encodes the monocyte-specific cytokine monocyte chemotactic protein 1 (MCP-
1). MCP-1 is a CC chemokine of 76 amino acids (~11 kDa) and is chemotactic for monocytes and
basophils but not neutrophils and eosinophils. MCP-1 is expressed by smooth muscle cells (SMC),
macrophages, endothelial cells, keratinocytes and fibroblasts in response to inflammatory stimuli such
as interleukin 1β and tumor necrosis factor α. MCP-1 has been implicated in a variety of inflammatory
processes, including inflammatory bowel disease, rheumatoid arthritis, asthma, nephritis, and parasitic
and viral infections. MCP-1 antigen is not detected in the endothelium or SMC of normal arteries.
MCP-1 has also been shown to exhibit biological activities other than chemotaxis. It can induce the
proliferation and activation of killer cells known as CHAK (CC-Chemokine-activated killer)
MCP-1 signals via the CCR2 receptor, and is critical for aneurysm formation because of its stability to
recruit leukocytes. These leukocytes produce extracellular matrix-degrading MMPs, thereby inductin
aortic remodelling and dilatation. Interleukin-6 is also involved in this amplification loop accelerating
vascular inflammation. MCP-/- mice display significantly delayed wound re-epithelialization, and also
delayed wound angiogenesis.
Frozen sections, Western blot
F: Sections (6 μm) were fixed with 4% PFA, blocked using 0.1% Triton-X and 5% serum for 15min at 37°C or o/n 4 °C and then incubated with 2 μg/ml antibody for 2h at 37°C.
W: Samples electrophoresed on 15% SDS-PAGE were blotted on nitrocellulose and blocked with PBS/5% low fat dry milk. The blot was incubated with antibody (0.8 μg/ml) for 20min at RT.
IHC-F: detection of MCP-1 in mouse testis. The antibody has been used in a 10x dilution, the magnification was 200x.
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.