Home \ Antibodies \ S100A9, Mouse, mAb MU14-2A5, biotinylated
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Catalog # HM1102BT-50UG

S100A9, Mouse, mAb MU14-2A5, biotinylated

276,00

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The monoclonal antibody MU14-2A5 recognizes S100A9. The calcium-binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. MRP14 is co-expressed with human MRP8, a homologue protein in myeloid cells, and plays an essential role in calcium dependent functions during inflammation. This role includes the activation of Mac-1, the β2 integrin which is involved in neutrophil adhesion to endothelial cells. MRP14 is distinguished from other S100 member proteins by its long C-terminal region.
The expression of MRP-8 (S100A8) and MRP-14 (S100A9) is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. In inflammatory conditions small venueles stain with both anti-S100A8 and S100A9. The staining of the two subunits is always coincident. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils.

Application
Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Paraffin sections, Western blot

Application Notes
W: A reduced sample treatment and SDS-Page was used. The band size is 13-15 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
Mouse bone marrow cell extracts

Product type
Monoclonal antibodies
Quantity
50 µg
Formulation
0.5 ml (100 μg/ml) 0.2 μm protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Conjugate
Biotin
Immunogen
Complete mouse S100A9 protein
Isotype
Rat IgG1
Species
Mouse
Cross reactivity
Rabbit – No
Alias
Migration inhibitory factor-related protein 14, MRP 14, Calgranulin B<br> - Gene name: S100a9, Cagb, Mrp14
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Gastroenterology

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