Home \ Antibodies \ TNF-RI, Human, mAb H398, biotinylated
Catalog # HM2020BT-50UG

TNF-RI, Human, mAb H398, biotinylated

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The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha.
TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.

Application
Flow cytometry, Functional studies, Immuno assays, Immuno precipitation, Western blot

Application Notes
W: Reduced sample conditions were used. A band size of ~50-60 kDa is expected.
FC: Antibody H398 can be used for extracellular staining.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. The antibody competes with TNF-alpha.

Positive Control
THP-1 cells

Product type
Monoclonal antibodies
Amount
50 µg
Formulation
0.5 ml (100 µg/ml) 0.2 μm filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Conjugate
Biotin
Immunogen
Affinity-purified receptor material from HL60 cells containing ~9 μg actively binding receptor (Ref.1).
Isotype
Mouse IgG2a
Species
Human
Cross reactivity
Rat – Yes
Alias
CD120a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, p55, p60, TNFR-1<br> - Gene name: TNFRSF1A, TNFAR, TNFR1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use or derivation of this product.
Disease
Autoimmunity, Gastroenterology

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