Home \ Antibodies \ TNF-alpha, Human, mAb T1, biotinylated
Catalog # HM2024BT-50UG

TNF-alpha, Human, mAb T1, biotinylated

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The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody HM2026, which recognizes soluble, membrane and receptor bound TNF-alpha.

Application
Flow cytometry, Immuno assays, Western blot

Use
For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Product type
Monoclonal antibodies
Amount
50 µg
Formulation
0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Conjugate
Biotin
Isotype
Mouse IgG1
Species
Human
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Disease
Autoimmunity, Gastroenterology
Protocol
Protocol Western Blot
59 kb
Protocol SDS Page
57 kb
Protocol Sandwich ELISA
49 kb
Protocol Flow Cytometry
58 kb
CoA-TDS HM1067-HM2099
HM2024BT-50UG
89 kb
Safety Data Sheet
SDS - HM2024BT-50UG
52 kb

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