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Catalog # HM2068BT-50UG

TLR4, Human, mAb HTA125, biotinylated

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Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. The HTA125 monoclonal antibody reacts preferentially, especially in flow cytometry, with human TLR4 (CD284) that is associated with MD-2. HTA125 is a TLR4 (CD284) function-blocking antibody that is useful for studies on the role of TLR4 (CD284) as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to precipitate TLR4 (100 kDa). The antibody HTA125 is cross reactive with canine, cynomolgus monkey, rhesus monkey and marmoset monkey.

Immuno fluorescence, Immuno precipitation, Western blot

Application Notes
FC: 300000 cells/50 µl were stained with 2  µg antibody for 30 minutes at 4 °C
FS: In cell culture 10  µg/ml
IF: Oregon green labeled HTA125 was used in FRAP measurements
IP: HTA125 (4 mg/ml) coupled to Sepharose 4FF beads was added to cell lysate and incubated for 2 hours at 4 °C
W: 20 mg protein was analyzed on SDS-PAGE and transferred to nitrocellulose. Blot was blocked with TBS/5% dry milk/0.1% tween-20

For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control

Product type
Monoclonal antibodies
50 µg
0.5 ml (100 µg/ml) 0.2 µm filtered biotinylated antibody solution in PBS, containing 0.02% chlooracetamide and 0.1% bovine serum albumin.
BALB/c mice were immunized with the Ba/F3 line expressing TLR4
Mouse IgG2a
Cross reactivity
Canine – Yes, Monkey – Yes
TLR4, CD284, ARMD10
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.
Infectious diseases

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