Home \ Antibodies \ CD36, Human, mAb FA6-152, biotinylated
Catalog # HM2122BT-50UG

CD36, Human, mAb FA6-152, biotinylated

238,00

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Monoclonal antibody FA6-152 recognizes human CD36 (88-kDa), a cell surface class B scavenger
receptor, also known as thrombospondin receptor CD36 is a heavily N-glycosylated transmembrane
protein of ~88 kDa with two short intracellular domains and a large extracellular domain. The protein is
sensitive for neuroaminidase, resulting in a shift from 88 to 85 kDa. CD36 is expressed on platelets,
mature monocytes and macrophages, microvascular endothelial cells, mammary endothelial cells,
during stages of erythroid cell development and on some macrophage derived dendritic cells. The
antibody recognizes adult and fetal monocytes, platelets and reticulocytes, but doesn’t stain
lymphocytes and granulocytes. Reactivity has also been found in small intestine, kidney, liver and
thyroid. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR
(peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within
lipid rafts, which facilitates its association with receptors, signaling and adaptor molecules. It is a
receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been implicated in
many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose
metabolism. Several in vivo models support the role of the thrombospondin / CD36 system in
angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major
receptor for P. falciparum-infected red blood cells. CD36 is associated with Src-family kinases and
with the integrins α3β1 and α6β1. Recently, CD36 has been identified as a protein that is required for
toll like receptor (TLR2) recognition of di-acylated bacterial lipopeptides and lipoteichoic acid4.
Furthermore, CD36 has been shown to function as phagocytic receptor for apoptotic cells. Many
different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a
structure-based domain rather than specific contact residues. Monoclonal antibody FA6-152 blocks
the biological activity of CD36 by blocking collagen/thrombospondin binding. The antibody
agglutinates fetal but not adult erythrocytes.

Application
Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Immuno precipitation

Application Notes
F: Tissue embedded in tissue-tek (for instance aortic tissue) followed by freezing in liquid nitrogen; 7-8  µm sections; air-dried; aceton-fixed; 10 % NGS as block (Ref 4).
FC: Antibody FA6-152 stains the extracellular domain of CD36. Unfixed cells;2  µg per 100.000 cells. Positive on granulocytes (Ref 1).
FS: Platelet aggregation and secretion was induced by; 1 µg/ml antibody (Ref 2).
IA: 10  µg/ml antibody as coat diluted in Tris-buffered saline ; 100  µm;l/well; o/n at RT (Ref 3).
IF: unfixed cells were incubated for 30 minutes at 4  °C followed by a secondary FITC polyclonal antibody; one-minute methanol fixation before analysis (Ref 1).
IP: 88 kDa sialoglycoprotein in platelets; 85 and 88 kDa in HEL cells. 10  µg antibody/200  µg protein

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Positive Control
THP-1 cells

Product type
Monoclonal antibodies
Quantity
50 µg
Formulation
0.5 ml (100 μg/ml) 0.2 μm filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Conjugate
Biotin
Immunogen
20-Weeks-old fetal erythrocytes
Isotype
Mouse IgG1
Species
Human
Alias
GPIV, Fatty acid translocase (FAT), platelet glycoprotein 4, PAS IV, Platelet collagen receptor, thrombospondin receptor</br> - Gene name: CD36
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Cardiology and metabolism, Infectious diseases

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