Home \ Antibodies \ Caspase-9, Human, mAb 3-20
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Catalog # HM2342

Caspase-9, Human, mAb 3-20

333,00

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The caspase-9 antibody 3-20 recognizes caspase-9, a member of the cysteine-aspartic acid protease (caspase) family. It is widely expressed and is particularly important during development. Human pro-caspase-9 is a 46 kDa, 416 amino acid (aa) protein and contains one CARD region (aa 192) and catalytic residues at His237 and Cys287. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. The caspase 9 protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Application
Immuno precipitation, Western blot

Application Notes
W: A reduced and non-reduced sample treatment and SDS-Page was used. The band size is ~45 kDa.

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Quantity
100 µg
Formulation
0.1 ml (1 mg/ml) 0.2 μm filtered antibody solution in PBS, containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunogen
Synthetic peptide
Isotype
Mouse IgG1
Species
Human
Cross reactivity
Mouse – Yes, Rat – Yes
Alias
Cysteineaspartic acid protease 9, Casp9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF3, Mch6, ICE-like apoptotic protease 6, ICELAP6 </br> - Gene name: CASP9, MCH6
Storage and stability
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
CoA-TDS HM2300-HM2400
HM2342
82 kb

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