This polyclonal antibody is directed against the interleukin 12 (IL-12) subunit IL12B. IL-12 is a key immunoregulatory cytokine that coordinates innate and adaptive immune responses. IL12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit IL12B, and a 35 kD subunit IL12A. IL12 is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. It acts on T and natural killer cells, and has a broad array of biological activities. The main role of IL-12 is activation of Interferon gamma ( IFN-gamma) production by triggering the Jak-STAT signaling pathway. Overexpression of the IL12 gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. IL-12 is also linked to autoimmunity. Promoter polymorphisms in the IL12 gene have been reported to be associated with the severity of atopic and non-atopic asthma in children.
Immuno assays, Paraffin sections, Western blot
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.