Home \ Antibodies \ JAM-A, Mouse, mAb BV12, FITC
FC in bEnd3 cells
Catalog # HM1050F

JAM-A, Mouse, mAb BV12, FITC

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The monoclonal antibody BV12 recognizes junctional adhesion molecule-A (JAM-A), also known as JAM-1 and the mouse platelet F11-Receptor (F11R), is a cell adhesion molecule (CAM). JAM-A is a member of the immunoglobulin superfamily found on the surface of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-B (VE-JAM or JAM-3) and JAM-C (JAM-2) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. In human JAM-A plays a role in platelet aggregation, secretion, adhesion and spreading.

Application
Flow cytometry, Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes
IHC-F: sections were air-dried overnight, fixed in acetone for 10 minutes, and incubated with 10% normal rabbit serum. Sections were then incubated with different primary Ab’s (Ref.3).
IP: Confluent monolayers of H5V cells were washed twice with PBS and then lysed for 30 min with lysis buffer. In some experiments, before cell lysis, cells were incubated for 2 h at room temperature in PBS with 5 mM bis(sulfosuccinimidyl) suberate (Ref.4).

Use
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
Amount
100 µg, 20 µg
Formulation
0.2 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated filtered antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Conjugate
FITC
Immunogen
H5V endothelial cells (Ref.1)
Isotype
Rat IgG2a
Species
Mouse
Cross reactivity
Human – No
Alias
Junctional adhesion molecule A, Junctional adhesion molecule 1, JAM-1, CD321<br> - Gene name: F11r, Jam1, Jcam, Jcam1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year.
Precautions
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Disease
Cardiology and metabolism, Tumor immunology
Protocol
Protocol Sandwich ELISA
49 kb
Protocol Immuno Fluorescence
39 kb
Protocol Flow Cytometry
58 kb
CoA-TDS HM1000-HM1066
HM1050F-100UG
96 kb
HM1050F-20UG
96 kb

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