JAM-A, Mouse, mAb BV12, FITC
The monoclonal antibody BV12 recognizes junctional adhesion molecule-A (JAM-A), also known as JAM-1 and the mouse platelet F11-Receptor (F11R), is a cell adhesion molecule (CAM). JAM-A is a member of the immunoglobulin superfamily found on the surface of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-B (VE-JAM or JAM-3) and JAM-C (JAM-2) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. In human JAM-A plays a role in platelet aggregation, secretion, adhesion and spreading.
IP: Confluent monolayers of H5V cells were washed twice with PBS and then lysed for 30 min with lysis buffer. In some experiments, before cell lysis, cells were incubated for 2 h at room temperature in PBS with 5 mM bis(sulfosuccinimidyl) suberate (Ref.4).
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