Home \ Antibodies \ JAM-A, Mouse, mAb BV12, FITC
FC in bEnd3 cells
Catalog # HM1050F

JAM-A, Mouse, mAb BV12, FITC

Please choose the quantity of items you wish to add to your cart…
Find distributor

The monoclonal antibody BV12 recognizes junctional adhesion molecule-A (JAM-A), also known as JAM-1 and the mouse platelet F11-Receptor (F11R), is a cell adhesion molecule (CAM). JAM-A is a member of the immunoglobulin superfamily found on the surface of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-B (VE-JAM or JAM-3) and JAM-C (JAM-2) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. In human JAM-A plays a role in platelet aggregation, secretion, adhesion and spreading.

Flow cytometry, Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot

Application Notes
IHC-F: sections were air-dried overnight, fixed in acetone for 10 minutes, and incubated with 10% normal rabbit serum. Sections were then incubated with different primary Ab’s (Ref.3).
IP: Confluent monolayers of H5V cells were washed twice with PBS and then lysed for 30 min with lysis buffer. In some experiments, before cell lysis, cells were incubated for 2 h at room temperature in PBS with 5 mM bis(sulfosuccinimidyl) suberate (Ref.4).

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Product type
Monoclonal antibodies
100 µg, 20 µg
0.2 ml (100 μg/ml) 0.2 μm protein G purified FITC conjugated filtered antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
H5V endothelial cells (Ref.1)
Rat IgG2a
Cross reactivity
Human – No
Junctional adhesion molecule A, Junctional adhesion molecule 1, JAM-1, CD321<br> - Gene name: F11r, Jam1, Jcam, Jcam1
Storage and stability
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year.
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.
Cardiology and metabolism, Tumor immunology

Calculate your ELISA data easily

With the ELISA calculator you can easily calculate ELISA data. Assayfit Pro helps to perform curve fitting. The calculator generates advanced reports, fit graph, fit parameters and goodness of fit are shown.

Latest Hycult Biotech news

  • New Human Complement Pathway Assays
    We are very proud of our newly developed human classical and alternative complement pathway assays. They are produced in response to a growing demand for quantitative investigation of complement inhibitors or regulators at lower sample dilutions. This development aims to address the issue of false negative results, enabling more accurate and reliable analysis of complement… Read more: New Human Complement Pathway Assays
  • Navigating the pitfalls in complement analysis
    Our colleague Erik Toonen shared his experience on how to analyze complement at the Complement-based Drug Development Summit in Boston in September 11-13th, 2023. He showed valuable insights on analyzing complements. For accurate complement analysis, it is important that not only the correct technique is used but also that pre-analytical sample handling is performed in… Read more: Navigating the pitfalls in complement analysis
  • NEW Human C3d ELISA
    We are happy to introduce our new ELISA (cat.# HK3017) designed to detect human C3d. What sets this assay apart is its utilization of a distinctive neo-epitope capture antibody that selectively targets a specific C3d region of the alpha chain, which is not available in other C3 variants. This way it specifically distinguishes C3d from native C3… Read more: NEW Human C3d ELISA
Contact us