The polyclonal antibody recognizes mouse LPLUNC1 (Long palate, lung and nasal epithelium clone 1). LPLUNC1, also known as BPI fold-containing family member B1 (BPIFB1), is a protein present in the trachea, the epithelium and submucosal glands of larger airways and some smaller airways. It is also found in minor mucosal glands of the nasal cavity and more abundantly in minor, compared with major, salivary glands. Currently, the function of LPLUNC is unknown. It may be involved in the innate immune
response to bacterial exposure in the mouth, nasal cavities, and lungs. Recently, a significant association between a specific LPLUNC1 SNP (single nucleotide polymorphism), located in the promoter region of the gene, and susceptibility to infection by the cholera-causing bacterium Vibrio cholera has been identified. However, its precise role in susceptibility to infection and/or the development of disease needs to be further elucidated.
Immuno fluorescence, Paraffin sections, Western blot
W: A reduced sample treatment and SDS-Page was used. The band size is 50 kDa (Ref.2).
IHC-P: Tissue sections were pretreated with tri-sodium citrate buffer for 8 minutes in a microwave oven
followed by rinsing in PBS. Tissue sections were fixed in 3 % H2O2 in methanol for 20 minutes (Ref.1)
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.